Cell-to-cell variability of gene expression in clonal populations of mammalian cells is
ubiquitous. However, because molecular biologists habitually assume uniformity of the cell …

Single-cell measurements and lineage-tracing experiments are revealing that phenotypic
cell-to-cell variability is often the result of deterministic processes, despite the existence of …

The development of drug resistance, the prime cause of failure in cancer therapy, is
commonly explained by the selection of resistant mutant cancer cells. However, dynamic …

In this chapter we have presented mathematical modeling approaches to biological pattern
formation. While reaction-diffusion models are appropriate to describe the spatio-temporal …

We have developed a new mass spectrometry (MS) technology, the Single-probe MS,
capable of real-time, in situ metabolomic analysis of individual living cells. The Single-probe …

The CRISPR/Cas9 system has enabled efficient modification of genes in a variety of cellular
systems for studying phenotypic effects of genetic perturbations. However, with this …

Natural systems are, almost by definition, heterogeneous: this can be either a boon or an
obstacle to be overcome, depending on the situation. Traditionally, when constructing …

The development of human-induced pluripotent stem cells (hiPSCs) has made possible
patient-specific modeling across the spectrum of human disease. Here, we discuss recent …

Chinese hamster ovary (CHO) cells are the preferred cell factory for the production of
therapeutic glycoproteins. Although efforts primarily within bioprocess optimization have led …

Recent discoveries suggest that cells of a clonal population often display multiple metabolic
phenotypes at the same time. Motivated by the success of mass spectrometry (MS) in the …