The oligosaccharide substrate for the N-linked protein glycosylation is assembled at the
membrane of the endoplasmic reticulum. Dolichyl pyrophosphate serves as a carrier in this …

Just a few decades ago, the saccharides bound to glycoproteins were considered little more
than an irritation. They increased the difficulty of purifying and characterizing proteins …

In Saccharomyces cerevisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for
endoplasmic reticulum-to-Golgi protein transport. By exploiting a GAL10-YPT1 fusion to …

Yeast sec62 mutant cells are defective in the translocation of several secretory precursor
proteins into the lumen of the endoplasmic reticulum (Rothblatt et al., 1989). The deficiency …

Glycosyl phosphatidylinositol (GPI) anchoring, N glycosylation, and O mannosylation of
protein occur in the rough endoplasmic reticulum and involve transfer of precursor structures …

It has been shown previously that defects in the essential GTP-binding protein, Ypt1p, lead
to a block in protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus in …

CRISPR-Cas9 functional genomic screens uncover gene targets linked to various
phenotypes for metabolic engineering with remarkable efficiency. However, these genome …

Asparagine-linked glycosylation is initiated by the synthesis of N-
acetylglucosaminylpyrophosphoryl dolichol (GlcNAc2-PP-dolichol), which is extended by a …

SEC62 is required for the import of secretory protein precursors into the endoplasmic
reticulum (ER) of Saccharomyces cerevisiae. The DNA sequence of SEC62 predicts a 32 …

The following study describes the discovery of a new inherited metabolic disorder, dolichol
kinase (DK1) deficiency. DK1 is responsible for the final step of the de novo biosynthesis of …