Chemistry, University of Illinois at Chicago, Chicago, IL, USA. The evaluation of nucleotide
photoionization threshold energies provides useful information for understanding …

We describe a tethered multifluorophore motion assay based on DNA origami for revealing
bimolecular reaction kinetics on the single-molecule level. Molecular binding partners may …

Abstract Tethered Particle Motion (TPM) is a versatile in vitro technique for monitoring the
conformations a linear macromolecule, such as DNA, can exhibit. The technique involves …

Protein-mediated DNA looping is fundamental to gene regulation and such loops occur
stochastically in purified systems. Additional proteins increase the probability of looping, but …

Supercoiling can alter the form and base pairing of the double helix and directly impact
protein binding. More indirectly, changes in protein binding and the stress of supercoiling …

RNA polymerases must transit through protein roadblocks to produce full-length transcripts.
Here we report real-time measurements of Escherichia coli RNA polymerase passing …

The range of motion of a micron-sized bead tethered by a single polymer provides a
dynamic readout of the effective length of the polymer. The excursions of the bead may …

Protein-mediated DNA looping is ubiquitous in chromatin organization and gene regulation,
but to what extent supercoiling or nucleoid associated proteins promote looping is poorly …

This work investigates the deposition of polystyrene particles onto cross-linked
polydimethylsiloxane (PDMS) substrates by using an impinging jet flow cell for different …

One of the most important biological processes is the dynamic folding and unfolding of
deoxyribonucleic acid (DNA). The folding process is crucial for DNA to fit within the …