Transcriptional silencing in Saccharomyces cerevisiae occurs at several genomic sites
including the silent mating-type loci, telomeres, and the ribosomal DNA (rDNA) tandem …

Chromatin is generally classified as euchromatin or heterochromatin, each with distinct
histone modifications, compaction levels, and gene expression patterns. Although the …

Exogenous pathway optimization and chassis engineering are two crucial methods for
heterologous pathway expression. The two methods are normally carried out step-wise and …

Background Classical studies on position effect variegation in Drosophila have
demonstrated the existence of bi-modal Active/Silent state of the genes juxtaposed to …

The genome is packaged and organized in an ordered, nonrandom manner, and specific
chromatin segments contact nuclear substructures to mediate this organization. tRNA genes …

Background Synthetic algal–fungal and algal–bacterial cultures have been investigated as a
means to enhance the technological applications of the algae. This inclusion of other …

Multiple studies in Saccharomyces cerevisiae have measured the levels of gene silencing
by inserting the URA3 gene at various loci and selecting against URA3-expressing cells by …

Hypomorphic mutations are a valuable tool for both genetic analysis of gene function and for
synthetic biology applications. However, current methods to generate hypomorphic …

How alternative chromatin-based regulatory states can be made stable and heritable in
order to provide robust epigenetic memory is poorly understood. Here, we develop a …

Transcriptional silencing in Saccharomyces cerevisiae is a persistent and highly stable form
of gene repression. It involves DNA silencers and repressor proteins that bind nucleosomes …