High-throughput methods for genome editing: the more the better

Y Huang, M Shang, T Liu, K Wang - Plant Physiology, 2022 - academic.oup.com
During the last decade, targeted genome-editing technologies, especially clustered
regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) …

Annual banned‐substance review: analytical approaches in human sports drug testing

M Thevis, T Kuuranne, H Geyer… - Drug testing and …, 2014 - Wiley Online Library
Monitoring the misuse of drugs and the abuse of substances and methods potentially or
evidently improving athletic performance by analytical chemistry strategies is one of the …

A next-generation sequencing method for gene doping detection that distinguishes low levels of plasmid DNA against a background of genomic DNA

EN de Boer, PE van der Wouden, LF Johansson… - Gene therapy, 2019 - nature.com
Gene doping confers health risks for athletes and is a threat to fair competition in sports.
Therefore the anti-doping community has given attention on its detection. Previously …

Microfluidic quantitative PCR detection of 12 transgenes from horse plasma for gene doping control

T Tozaki, A Ohnuma, M Kikuchi, T Ishige, H Kakoi… - Genes, 2020 - mdpi.com
Gene doping, an activity which abuses and misuses gene therapy, is a major concern in
sports and horseracing industries. Effective methods capable of detecting and monitoring …

Droplet digital PCR detection of the erythropoietin transgene from horse plasma and urine for gene-doping control

T Tozaki, A Ohnuma, M Takasu, M Kikuchi, H Kakoi… - Genes, 2019 - mdpi.com
Indiscriminate genetic manipulation to improve athletic ability is a major threat to human
sports and the horseracing industry, in which methods involving gene-doping, such as …

Establishment of two quantitative nested qPCR assays targeting the human EPO transgene

EWI Neuberger, I Perez, C Le Guiner, D Moser, T Ehlert… - Gene therapy, 2016 - nature.com
For ethical and safety reasons it is critical to develop easily implemented assays with high
sensitivity and specificity for gene doping surveillance. Two nested quantitative real-time …

A duplex qPCR assay for human erythropoietin (EPO) transgene to control gene doping in horses

HW Cheung, KS Wong, VYC Lin… - Drug testing and …, 2021 - Wiley Online Library
The misuse of genetic manipulation technology to enhance athletic performance is termed
gene doping which is prohibited in human sports, horseracing, and equestrian sports …

Gene doping and genomic science in sports: where are we?

S López, J Meirelles, V Rayol, G Poralla, N Woldmar… - Bioanalysis, 2020 - Taylor & Francis
The misuse of sport-related gene transfer methods in elite athletes is a real and growing
concern. The success of gene therapy in the treatment of hereditary diseases has been most …

EPO transgene detection in dried blood spots for antidoping application

A Marchand, I Roulland, F Semence… - Drug Testing and …, 2021 - Wiley Online Library
The modification of gene expression to treat diseases is a field of research with exponential
growth. As doping in sport closely follows emerging therapies, a surveillance of the …

Ultrasensitive and rapid detection for multiple dopings in saliva and urine using surface-enhanced Raman spectroscopy

D Feng, SS Xu, BY Wen, M Kathiresan… - Spectroscopy …, 2023 - Taylor & Francis
Abuse of dopings is one of the serious issues affecting competitive sports globally.
Traditional means of analysis for doping detection face the challenges of costly, bulky, and …