Fluorescence microscopy is one of the most powerful tools for elucidating the cellular
functions of proteins and other molecules. In many cases, the function of a molecule can be …

ImageJ will celebrate its tenth anniversary in September of this year. These past 10 years
have seen the Javabased open-source software mature into an invaluable laboratory tool. In …

Nuclear speckles are dynamic membraneless bodies located in the cell nucleus. They
harbor RNAs and proteins, many of which are splicing factors, that together display complex …

A key goal of biology is to relate the expression of specific genes to a particular cellular
phenotype. However, current assays for gene expression destroy the structural context. By …

Models for replication and transcription often display polymerases that track like locomotives
along their DNA templates. However, recent evidence supports an alternative model in …

Nucleolar segregation is observed under some physiological conditions of transcriptional
arrest. This process can be mimicked by transcriptional arrest after actinomycin D treatment …

BioImageXD puts open-source computer science tools for three-dimensional visualization
and analysis into the hands of all researchers, through a user-friendly graphical interface …

Polarity-dependent fluorescent probes are recently attracting interest for high-resolution cell
imaging. Following a stepwise rational approach, we prepared and tested a toolbox of new …

Genes on different chromosomes can be spatially associated in the nucleus in several
transcriptional and regulatory situations; however, the functional significance of such …

Using HeLa cells, we have developed methods to determine 1) the number of RNA
polymerases that are active at any moment, 2) the number of transcription sites, and 3) the …