Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
Wearable materials with embedded synthetic biology sensors for biomolecule detection
Integrating synthetic biology into wearables could expand opportunities for noninvasive
monitoring of physiological status, disease states and exposure to pathogens or toxins …
monitoring of physiological status, disease states and exposure to pathogens or toxins …
A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor
Advances in biosensor engineering have enabled the design of programmable molecular
systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer …
systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer …
A glucose meter interface for point-of-care gene circuit-based diagnostics
E Amalfitano, M Karlikow, M Norouzi, K Jaenes… - Nature …, 2021 - nature.com
Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors
with the potential to provide decentralized and low-cost molecular diagnostics. However, it …
with the potential to provide decentralized and low-cost molecular diagnostics. However, it …
Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria
Carbon-negative synthesis of biochemical products has the potential to mitigate global CO2
emissions. An attractive route to do this is the reverse β-oxidation (r-BOX) pathway coupled …
emissions. An attractive route to do this is the reverse β-oxidation (r-BOX) pathway coupled …
A multiplexed, electrochemical interface for gene-circuit-based sensors
The field of synthetic biology has used the engineered assembly of synthetic gene networks
to create a wide range of functions in biological systems. To date, gene-circuit-based …
to create a wide range of functions in biological systems. To date, gene-circuit-based …
Advances in cell‐free biosensors: principle, mechanism, and applications
L Zhang, W Guo, Y Lu - Biotechnology Journal, 2020 - Wiley Online Library
Synthetic biology has promoted the development of biosensors as tools for detecting trace
substances. In the past, biosensors based on synthetic biology have been designed on …
substances. In the past, biosensors based on synthetic biology have been designed on …
Synthetic biology goes cell-free
A Tinafar, K Jaenes, K Pardee - BMC biology, 2019 - Springer
Cell-free systems (CFS) have recently evolved into key platforms for synthetic biology
applications. Many synthetic biology tools have traditionally relied on cell-based systems …
applications. Many synthetic biology tools have traditionally relied on cell-based systems …