Critical challenges and advances in recombinant adeno‐associated virus (rAAV) biomanufacturing
Gene therapy is a promising therapeutic approach for genetic and acquired diseases
nowadays. Among DNA delivery vectors, recombinant adeno‐associated virus (rAAV) is one …
nowadays. Among DNA delivery vectors, recombinant adeno‐associated virus (rAAV) is one …
The state of technological advancement to address challenges in the manufacture of rAAV gene therapies
Current processes for the production of recombinant adeno-associated virus (rAAV) are
inadequate to meet the surging demand for rAAV-based gene therapies. This article reviews …
inadequate to meet the surging demand for rAAV-based gene therapies. This article reviews …
High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography
The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform
with several approved products that have marked therapeutic impact for patients. However …
with several approved products that have marked therapeutic impact for patients. However …
Stimulus-responsive assembly of nonviral nucleocapsids
Controlled assembly of a protein shell around a viral genome is a key step in the life cycle of
many viruses. Here we report a strategy for regulating the co-assembly of nonviral proteins …
many viruses. Here we report a strategy for regulating the co-assembly of nonviral proteins …
[HTML][HTML] Partial genome content within rAAVs impacts performance in a cell assay-dependent manner
B Troxell, SL Jaslow, IW Tsai, C Sullivan… - … Therapy Methods & …, 2023 - cell.com
Recombinant adeno-associated viruses (rAAVs) deliver DNA to numerous cell types.
However, packaging of partial genomes into the rAAV capsid is of concern. Although empty …
However, packaging of partial genomes into the rAAV capsid is of concern. Although empty …
Recent advances in upstream process development for production of recombinant adeno‐associated virus
Recombinant adeno‐associated virus (rAAV) is rapidly emerging as the preferred delivery
vehicle for gene therapies, with promising advantages in safety and efficacy. Key challenges …
vehicle for gene therapies, with promising advantages in safety and efficacy. Key challenges …
The downstream bioprocess toolbox for therapeutic viral vectors
Viral vectors are poised to acquire a prominent position in modern medicine and
biotechnology owing to their role as delivery agents for gene therapies, oncolytic agents …
biotechnology owing to their role as delivery agents for gene therapies, oncolytic agents …
Production of adeno‐associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities
In recent years, the use of adeno‐associated viruses (AAVs) as vectors for gene and cell
therapy has increased, leading to a rise in the amount of AAV vectors required during pre …
therapy has increased, leading to a rise in the amount of AAV vectors required during pre …
Empty and full AAV capsid charge and hydrophobicity differences measured with single-particle AFM
Adeno-associated virus (AAV) is showing promise as a therapy for diseases that contain a
single-gene deletion or mutation. One major scale-up challenge is the removal of empty or …
single-gene deletion or mutation. One major scale-up challenge is the removal of empty or …
Critical assessment of purification and analytical technologies for enveloped viral vector and vaccine processing and their current limitations in resolving co-expressed …
A Do Minh, AA Kamen - Vaccines, 2021 - mdpi.com
Viral vectors and viral vaccines are invaluable tools in prevention and treatment of diseases.
Many infectious diseases are controlled using vaccines designed from subunits or whole …
Many infectious diseases are controlled using vaccines designed from subunits or whole …