[FeFe]-Hydrogenases: maturation and reactivity of enzymatic systems and overview of biomimetic models

JT Kleinhaus, F Wittkamp, S Yadav… - Chemical Society …, 2021 - pubs.rsc.org
While hydrogen plays an ever-increasing role in modern society, nature has utilized
hydrogen since a very long time as an energy carrier and storage molecule. Among the …

Enzymatic cascade reactions in biosynthesis

CT Walsh, BS Moore - Angewandte Chemie International …, 2019 - Wiley Online Library
Enzyme‐mediated cascade reactions are widespread in biosynthesis. To facilitate
comparison with the mechanistic categorizations of cascade reactions by synthetic chemists …

Crystal Structure of the [FeFe]-hydrogenase maturase HydE bound to complex-B

R Rohac, L Martin, L Liu, D Basu, L Tao… - Journal of the …, 2021 - ACS Publications
[FeFe]-hydrogenases use a unique organometallic complex, termed the H cluster, to
reversibly convert H2 into protons and low-potential electrons. It can be best described as a …

Proton Shuttle Mediated by (SCH2)2P═O Moiety in [FeFe]-Hydrogenase Mimics: Electrochemical and DFT Studies

LR Almazahreh, F Arrigoni, H Abul-Futouh… - ACS …, 2021 - ACS Publications
The synthesis, characterization, and protonation of [Fe2 (CO) 6 {(μ-SCH2) 2 (Et) P═ O}](1)
using the moderately strong acid CF3CO2H (p K aMeCN= 12.7) are reported. Digital …

From protein engineering to artificial enzymes–biological and biomimetic approaches towards sustainable hydrogen production

C Esmieu, P Raleiras, G Berggren - Sustainable Energy & Fuels, 2018 - pubs.rsc.org
Hydrogen gas is used extensively in industry today and is often put forward as a suitable
energy carrier due its high energy density. Currently, the main source of molecular hydrogen …

Semisynthetic maturation of [FeFe]-hydrogenase using [Fe 2 (μ-SH) 2 (CN) 2 (CO) 4] 2−: key roles for HydF and GTP

B Balci, RD O'Neill, EM Shepard, A Pagnier… - Chemical …, 2023 - pubs.rsc.org
Here we describe maturation of the [FeFe]-hydrogenase from its [4Fe–4S]-bound precursor
state by using the synthetic complex [Fe2 (μ-SH) 2 (CN) 2 (CO) 4] 2− together with HydF and …

A [4Fe–4S]-Fe(CO)(CN)-l-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly

G Rao, L Tao, DLM Suess, RD Britt - Nature chemistry, 2018 - nature.com
Biosynthesis of the [FeFe] hydrogenase active site (the'H-cluster') requires the interplay of
multiple proteins and small molecules. Among them, the radical S-adenosylmethionine …

The binuclear cluster of [FeFe] hydrogenase is formed with sulfur donated by cysteine of an [Fe(Cys)(CO)2(CN)] organometallic precursor

G Rao, SA Pattenaude, K Alwan… - Proceedings of the …, 2019 - National Acad Sciences
The enzyme [FeFe]-hydrogenase (HydA1) contains a unique 6-iron cofactor, the H-cluster,
that has unusual ligands to an Fe–Fe binuclear subcluster: CN−, CO, and an azadithiolate …

Artificial Maturation of the Highly Active Heterodimeric [FeFe] Hydrogenase from Desulfovibrio desulfuricans ATCC 7757

JA Birrell, K Wrede, K Pawlak… - Israel Journal of …, 2016 - Wiley Online Library
Hydrogenases catalyze the reduction of protons and oxidation of molecular hydrogen with
high turnover frequencies and low overpotentials under ambient conditions. The …

Cyanide ligand assembly by carbon atom transfer to an iron nitride

JL Martinez, HJ Lin, WT Lee, M Pink… - Journal of the …, 2017 - ACS Publications
The new iron (IV) nitride complex PhB (iPr2Im) 3Fe N reacts with 2 equiv of bis
(diisopropylamino) cyclopropenylidene (BAC) to provide PhB (iPr2Im) 3Fe (CN)(N2)(BAC) …