Highlights•Immune fitness in health and disease depends notably on the diversity of cellular
repertoires.•Single-cell sequencing is fostering the understanding of clonal diversity of T-cell …

Spatial transcriptomics technologies aim to advance gene expression studies by profiling
the entire transcriptome with intact spatial information from a single histological slide …

We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA
sequencing that has overcome many challenges hindering the development of scRNA-seq …

Recent years have witnessed rapid progress in the field of epitranscriptomics. Functional
interpretation of the epitranscriptome relies on sequencing technologies that determine the …

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method
with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire …

The mRNA cap structure is a major site of dynamic mRNA methylation. mRNA caps exist in
either the Cap1 or Cap2 form, depending on the presence of 2′-O-methylation on the first …

Abstract We present Light-Seq, an approach for multiplexed spatial indexing of intact
biological samples using light-directed DNA barcoding in fixed cells and tissues followed by …

Selfish, non-long terminal repeat (non-LTR) retroelements and mobile group II introns
encode reverse transcriptases (RTs) that can initiate DNA synthesis without substantial base …

In Escherichia coli and Salmonella, many genes silenced by the nucleoid structuring protein
H-NS are activated upon inhibiting Rho-dependent transcription termination. This response …

We describe a low-input RNase footprinting approach for the rapid quantification of
ribosome-protected fragments with as few as 1000 cultured cells. The assay uses a …