Critical review of NGS analyses for de novo genotyping multigene families

J Lighten, C Van Oosterhout, P Bentzen - Molecular Ecology, 2014 - Wiley Online Library
The genotyping of highly polymorphic multigene families across many individuals used to be
a particularly challenging task because of methodological limitations associated with …

amplisas: a web server for multilocus genotyping using next‐generation amplicon sequencing data

A Sebastian, M Herdegen, M Migalska… - Molecular Ecology …, 2016 - Wiley Online Library
Next‐generation sequencing (NGS) technologies are revolutionizing the fields of biology
and medicine as powerful tools for amplicon sequencing (AS). Using combinations of …

MHC genotyping of non-model organisms using next-generation sequencing: a new methodology to deal with artefacts and allelic dropout

S Sommer, A Courtiol, CJ Mazzoni - BMC genomics, 2013 - Springer
Abstract Background The Major Histocompatibility Complex (MHC) is the most important
genetic marker to study patterns of adaptive genetic variation determining pathogen …

Ultra‐deep Illumina sequencing accurately identifies MHC class IIb alleles and provides evidence for copy number variation in the guppy (Poecilia reticulata)

J Lighten, C Van Oosterhout… - Molecular Ecology …, 2014 - Wiley Online Library
We address the bioinformatic issue of accurately separating amplified genes of the major
histocompatibility complex (MHC) from artefacts generated during high‐throughput …

A review of molecular approaches for investigating patterns of coevolution in marine host–parasite relationships

G Froeschke, S von der Heyden - Advances in parasitology, 2014 - Elsevier
Parasites and their relationships with hosts play a crucial role in the evolutionary pathways
of every living organism. One method of investigating host–parasite systems is using a …

Neutral and selective processes shape MHC diversity in roe deer in Slovenia

E Buzan, S Potušek, L Duniš, B Pokorny - Animals, 2022 - mdpi.com
Simple Summary Disease prevention and appropriate wildlife management are among the
major challenges in wildlife conservation. In the present study, we made a first assessment …

Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland

Y Sugiyama, Y Nishita, GMJ Lansink, K Holmala… - PLOS …, 2022 - journals.plos.org
The wolverine (Gulo gulo) in Finland has undergone significant population declines in the
past. Since major histocompatibility complex (MHC) genes encode proteins involved in …

Lack of Spatial Immunogenetic Structure among Wolverine (Gulo gulo) Populations Suggestive of Broad Scale Balancing Selection

Y Rico, J Morris-Pocock, J Zigouris, JJ Nocera, CJ Kyle - PloS one, 2015 - journals.plos.org
Elucidating the adaptive genetic potential of wildlife populations to environmental selective
pressures is fundamental for species conservation. Genes of the major histocompatibility …

Phylogeography and Post-Glacial Recolonization in Wolverines (Gulo gulo) from across Their Circumpolar Distribution

J Zigouris, JA Schaefer, C Fortin, CJ Kyle - PloS one, 2013 - journals.plos.org
Interglacial-glacial cycles of the Quaternary are widely recognized in shaping
phylogeographic structure. Patterns from cold adapted species can be especially informative …

Lack of statistical power as a major limitation in understanding MHC‐mediated immunocompetence in wild vertebrate populations

A Gaigher, R Burri, LM San‐Jose, A Roulin… - Molecular …, 2019 - Wiley Online Library
Disentangling the sources of variation in developing an effective immune response against
pathogens is of major interest to immunoecology and evolutionary biology. To date, the link …