Optogenetics combines light and genetics to enable precise control of living cells, tissues,
and organisms with tailored functions. Optogenetics has the advantages of …

Since the neurobiological inception of optogenetics, light‐controlled molecular perturbations
have been applied in many scientific disciplines to both manipulate and observe cellular …

Inducible protein switches are currently limited for use in tissues and organisms because
common inducers cannot be controlled with precision in space and time in optically dense …

We describe single-component optogenetic probes whose activation dynamics depend on
both light and temperature. We used the BcLOV4 photoreceptor to stimulate Ras and …

Optogenetic tools are created to control RhoA GTPase, a central regulator of actin
organization and actomyosin contractility. RhoA GTPase, or its upstream activator …

Chemogenetic methods enabling the rapid translocation of specific proteins to the plasma
membrane (PM) in a single protein-single ligand manner are useful tools in cell biology. We …

We describe the efficient creation of single-component optogenetic tools for membrane
recruitment-based signaling perturbation using BcLOV4 technology. The workflow requires …

We report the construction of a single-component optogenetic Rac1 (opto-Rac1) to control
actin polymerization by dynamic membrane recruitment. Opto-Rac1 is a fusion of wildtype …

Eukaryotic protein kinases (EPKs) catalyze the transfer of a phosphate group onto another
protein in response to appropriate regulatory cues. In doing so, they provide a primary …

Only a few short decades have passed since the sequencing of GFP, yet the modern
repertoire of transgenically encoded optical tools implies an exponential proliferation of ever …