[HTML][HTML] Real-time PCR applied to study on plant pathogens: potential applications in diagnosis-a review

SM Mirmajlessi, E Loit, M Maend… - Plant Protection …, 2015 - agriculturejournals.cz
Quantitative real-time PCR (qPCR) technique incorporates traditional polymerase chain
reaction (PCR) efficiency with the production of a specific fluorescent signal, measuring the …

Promising perspectives for detection, identification, and quantification of plant pathogenic fungi and oomycetes through targeting mitochondrial DNA

T Kulik, K Bilska, M Żelechowski - International Journal of Molecular …, 2020 - mdpi.com
Fungi and oomycetes encompass many pathogens affecting crops worldwide. Their effective
control requires screening pathogens across the local and international trade networks …

Assessment of potato late blight from UAV-based multispectral imagery

J Rodriguez, I Lizarazo, F Prieto… - … and Electronics in …, 2021 - Elsevier
This paper evaluates a method for assessment and detection of potato late blight using UAV-
based multispectral imagery. Traditional methods of detection and mapping of late blight are …

Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles

AM Baetsen-Young, M Vasher, LL Matta… - Biosensors and …, 2018 - Elsevier
The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a
variety of diagnostic applications, with further diversification of synthesis match bio …

Loop‐mediated isothermal amplification for detection of the tomato and potato late blight pathogen, Phytophthora infestans

ZR Hansen, BJ Knaus, JF Tabima… - Journal of Applied …, 2016 - academic.oup.com
Aims To design and validate a colorimetric loop‐mediated isothermal amplification assay for
rapid detection of Phytophthora infestans DNA. Methods and Results Two sets of loop …

A rapid and economical method for efficient DNA extraction from diverse soils suitable for metagenomic applications

SG Devi, AA Fathima, S Radha, R Arunraj, WR Curtis… - PloS one, 2015 - journals.plos.org
A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for
environmental microbiology. The present work describes an improved method of DNA …

Development of Real-Time Isothermal Amplification Assays for On-Site Detection of Phytophthora infestans in Potato Leaves

M Si Ammour, GJ Bilodeau, DM Tremblay… - Plant …, 2017 - Am Phytopath Society
Real-time loop-mediated isothermal amplification (LAMP) and recombinase polymerase
amplification (RPA) assays were developed targeting the internal transcribed spacer 2 …

A Rapid, Equipment-Free Method for Detecting Phytophthora infestans in the Field Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay

X Lu, Y Zheng, F Zhang, J Yu, T Dai, R Wang… - Plant …, 2020 - Am Phytopath Society
Late blight, caused by the oomycete Phytophthora infestans, is a major constraint on the
production of potatoes and tomatoes as well as a constant threat to global food security. An …

Development of a new PCR assay and a recombinase-aided amplification based isothermal amplification coupled with lateral flow dipstick assay for potato late blight …

X Tang, H Li, W Huang, L Wang, Y Zhao, J Wang… - Crop Protection, 2023 - Elsevier
Late blight caused by Phytophthora infestans is the most devastating potato disease, yet leaf
infections are not reliably detected by current assays during the incubation phase of the …

The Use of qPCR Reveals a High Frequency of Phytophthora quercina in Two Spanish Holm Oak Areas

B Mora-Sala, M Berbegal, P Abad-Campos - Forests, 2018 - mdpi.com
The struggling Spanish holm oak woodland situation associated with Phytophthora root rot
has been studied for a long time. Phytophthora cinnamomi is considered the main, but not …