Uncovering the cis-regulatory code that governs when and how much each gene is
transcribed in a given genome and cellular state remains a central goal of biology. Here, we …

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

In animals, the sequences for controlling gene expression do not concentrate just at the
transcription start site of genes, but are frequently thousands to millions of base pairs distal …

The four-dimensional nucleome (4DN) consortium studies the architecture of the genome
and the nucleus in space and time. We summarize progress by the consortium and highlight …

Access to DNA is a prerequisite to the execution of essential cellular processes that include
transcription, replication, chromosomal segregation, and DNA repair. How the proteins that …

Over a decade ago the advent of high-throughput chromosome conformation capture (Hi-C)
sparked a new era of 3D genomics. Since then the number of methods for mapping the 3D …

The brain is one of the most complex living tissue types and is composed of an exceptional
diversity of cell types displaying unique functional connectivity. Single-cell RNA sequencing …

Nuclear bodies (NBs) are biomolecular condensates that participate in various cellular
processes and respond to cellular stimuli in the nucleus. The assembly and function of these …

Although often located at a distance from their target gene promoters, enhancers are the
primary genomic determinants of temporal and spatial transcriptional specificity in …

Mammalian genomes are organized and regulated through long-range chromatin
interactions. Structural loops formed by CCCTC-binding factor (CTCF) and cohesin fold the …