Native mass spectrometry (MS) involves the analysis and characterization of
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …

Charge detection mass spectrometry (CDMS) is a single-particle technique where the
masses of individual ions are determined from simultaneous measurement of their mass-to …

Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Hierarchical organization of integral membrane proteins (IMP) and lipids at the membrane is
essential for regulating myriad downstream signaling. A quantitative understanding of these …

Here we describe a detailed protocol for both data collection and interpretation with respect
to ion mobility–mass spectrometry analysis of large protein assemblies. Ion mobility is a …

Nucleic acids have been among the first targets for antitumor drugs and antibiotics. With the
unveiling of new biological roles in regulation of gene expression, specific DNA and RNA …

Native mass spectrometry (nMS) is evolving into a workhorse for structural biology. The
plethora of online and offline preparation, separation, and purification methods as well as …

Small molecules are known to stabilize membrane proteins and to modulate their function
and oligomeric state, but such interactions are often hard to precisely define. Here we …

Traditionally, mass spectrometry has been a powerful analytical method enabling the
structural analysis of small molecules, and later on peptides and proteins. With the advent of …

The growing number of applications to determine the stoichiometry, interactions and even
subunit architecture of protein complexes from mass spectra suggests that some general …