Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application
CF Cervellera, C Mazziotta, G Di Mauro… - Stem Cell Research & …, 2023 - Springer
Background Blood transfusions represent common medical procedures, which provide
essential supportive therapy. However, these procedures are notoriously expensive for …
essential supportive therapy. However, these procedures are notoriously expensive for …
Induction of enucleation in primary and immortalized erythroid cells
S Soboleva, K Miharada - International Journal of Hematology, 2022 - Springer
Enucleation is a crucial event during the erythropoiesis, implicating drastic morphologic and
transcriptomic/proteomic changes. While many genes deletion lead to failed or impaired …
transcriptomic/proteomic changes. While many genes deletion lead to failed or impaired …
Development of pathophysiologically relevant models of sickle cell disease and β-thalassemia for therapeutic studies
Ex vivo cellular system that accurately replicates sickle cell disease and β-thalassemia
characteristics is a highly sought-after goal in the field of erythroid biology. In this study, we …
characteristics is a highly sought-after goal in the field of erythroid biology. In this study, we …
JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis
S Metral, S Genetet, B Gamain, I Mouro-Chanteloup - Scientific Reports, 2024 - nature.com
New hematopoietic cell models have recently emerged through immortalization of CD34
cells to study and understand various molecular mechanisms of erythropoiesis. Here, we …
cells to study and understand various molecular mechanisms of erythropoiesis. Here, we …
Comprehensive analysis of microRNAs in human adult erythropoiesis
A Nath, J Rayabaram, S Ijee, A Bagchi, AD Chaudhury… - Cells, 2021 - mdpi.com
MicroRNAs (miRNAs) are small non-coding RNAs, which play an important role in various
cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to …
cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to …
Erythroid lineage-specific lentiviral RNAi vectors suitable for molecular functional studies and therapeutic applications
A Bagchi, N Devaraju, K Chambayil, V Rajendiran… - Scientific Reports, 2022 - nature.com
Numerous genes exert multifaceted roles in hematopoiesis. Therefore, we generated novel
lineage-specific RNA interference (RNAi) lentiviral vectors, H23B-Ery-Lin-shRNA and …
lineage-specific RNA interference (RNAi) lentiviral vectors, H23B-Ery-Lin-shRNA and …
Base editing of key residues in the BCL11A-XL-specific zinc finger domains derepresses fetal globin expression
V Rajendiran, N Devaraju, M Haddad, NS Ravi… - Molecular Therapy, 2024 - cell.com
BCL11A-XL directly binds and represses the fetal globin (HBG1/2) gene promoters, using 3
zinc-finger domains (ZnF4, ZnF5, and ZnF6), and is a potential target for β …
zinc-finger domains (ZnF4, ZnF5, and ZnF6), and is a potential target for β …
体外生产红细胞: 进展与挑战
王辉， 张进进， 陈立力， 邢颜超 - 协和医学杂志, 2024 - xhyxzz.pumch.cn
目前临床用血供不应求, 尽管自体输血等血液保护技术可部分缓解此类情况,
但红细胞作为主要的血液成分需求量巨大, 而体外生产安全的红细胞则可解决这一难题 …
但红细胞作为主要的血液成分需求量巨大, 而体外生产安全的红细胞则可解决这一难题 …
Efficient deletion of microRNAs using CRISPR/Cas9 with dual guide RNAs
S Ijee, K Chambayil, AD Chaudhury… - Frontiers in Molecular …, 2024 - frontiersin.org
MicroRNAs (miRNAs) are short non-coding RNAs that play crucial roles in gene regulation,
exerting post-transcriptional silencing, thereby influencing cellular function, development …
exerting post-transcriptional silencing, thereby influencing cellular function, development …
Immortalised chronic myeloid leukemia (CML) derived mesenchymal stromal cells (MSCs) line retains the immunomodulatory and chemoprotective properties of CML …
ESB Benjamin, E Vinod, RSS Illangeswaran… - Cellular …, 2024 - Elsevier
Despite the success of Tyrosine kinase inhibitors (TKIs) in treating chronic myeloid leukemia
(CML), leukemic stem cells (LSCs) persist, contributing to relapse and resistance. CML …
(CML), leukemic stem cells (LSCs) persist, contributing to relapse and resistance. CML …