Nowadays the practice of therapeutic drug monitoring, consisting in the measurement of
drugs concentrations in biological matrices in order to guide possible posological …

LC–MS is becoming a standard for many applications, thanks to high sensitivity and
selectivity; nevertheless, some issues are still present, particularly due to matrix effect (ME) …

The developments in mass spectrometry (MS) in the past few decades reveal the power and
versatility of this technology. MS methods are utilized in routine analyses as well as research …

There are few fields of medicine in which the individualisation of medicines is more
important than in the area of oncology. Under‐dosing can have significant ramifications due …

Sample preparation frequently is considered the most critical stage of the analytical
workflow. It affects the analytical throughput and costs; moreover, it is the primary source of …

Tacrolimus (TAC) is the cornerstone of immunosuppressive therapy in liver transplantation.
This study aimed at elucidating the interplay between pharmacogenetic determinants of TAC …

Background: Therapeutic drug monitoring (TDM) of the 2 calcineurin inhibitors (CNIs),
tacrolimus (TAC) and cyclosporin A, has resulted in improvements in the management of …

Introduction After kidney transplantation, rejection and drug-related toxicity occur despite
tacrolimus whole-blood pre-dose concentrations ([Tac] blood) being within the target range …

Aims Tacrolimus is a critical dose drug and to avoid under‐and overexposure, therapeutic
drug monitoring is standard practice. However, rejection and drug‐related toxicity occur …

Methods: PBMCs were isolated from whole blood by gradient centrifugation. After methanol-
based extraction, liquid chromatography with tandem mass spectrometry was used to …