DNA replication in eukaryotic cells initiates from large numbers of sites called replication
origins. Initiation of replication from these origins must be tightly controlled to ensure the …

Errors occurring during DNA replication can result in inaccurate replication, incomplete
replication, or re-replication, resulting in genome instability that can lead to diseases such as …

The MCM motor of the replicative helicase is loaded onto origin DNA as an inactive double
hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S …

DNA replication begins with the assembly of pre-replication complexes (pre-RCs) at
thousands of DNA replication origins during the G1 phase of the cell cycle. At the G1–S …

The activation of eukaryotic origins of replication occurs in temporally separated steps to
ensure that chromosomes are copied only once per cell cycle. First, the MCM helicase is …

Diploid and stable karyotypes are associated with health and fitness in animals. By contrast,
whole-genome duplications—doublings of the entire complement of chromosomes—are …

Assembly of the CMG (CDC-45–MCM-2-7–GINS) helicase is the key regulated step during
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …

The eukaryotic replisome is a molecular machine that coordinates the Cdc45-MCM-GINS
(CMG) replicative DNA helicase with DNA polymerases α, δ, and ε and other proteins to …

The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

Interstrand crosslinks (ICLs) are highly toxic DNA lesions that prevent transcription and
replication by inhibiting DNA strand separation. Agents that induce ICLs were one of the …