The necessity of and strategies for improving confidence in the accuracy of western blots

R Ghosh, JE Gilda, AV Gomes - Expert review of proteomics, 2014 - Taylor & Francis
Western blotting is one of the most commonly used laboratory techniques for identifying
proteins and semi-quantifying protein amounts; however, several recent findings suggest …

Tubulin or not tubulin: heading toward total protein staining as loading control in western blots

CP Moritz - Proteomics, 2017 - Wiley Online Library
Western blotting is an analytical method widely used for detecting and (semi‐) quantifying
specific proteins in given samples. Western blots are continuously applied and developed …

Required growth facilitators propel axon regeneration across complete spinal cord injury

MA Anderson, TM O'Shea, JE Burda, Y Ao, SL Barlatey… - Nature, 2018 - nature.com
Transected axons fail to regrow across anatomically complete spinal cord injuries (SCI) in
adults. Diverse molecules can partially facilitate or attenuate axon growth during …

Astrocyte scar formation aids central nervous system axon regeneration

MA Anderson, JE Burda, Y Ren, Y Ao, TM O'Shea… - Nature, 2016 - nature.com
Transected axons fail to regrow in the mature central nervous system. Astrocytic scars are
widely regarded as causal in this failure. Here, using three genetically targeted loss-of …

[HTML][HTML] Fetal manipulation of maternal metabolism is a critical function of the imprinted Igf2 gene

J Lopez-Tello, HEJ Yong, I Sandovici, GKC Dowsett… - Cell Metabolism, 2023 - cell.com
Maternal-offspring interactions in mammals involve both cooperation and conflict. The fetus
has evolved ways to manipulate maternal physiology to enhance placental nutrient transfer …

A defined methodology for reliable quantification of Western blot data

SC Taylor, T Berkelman, G Yadav, M Hammond - Molecular biotechnology, 2013 - Springer
Chemiluminescent western blotting has been in common practice for over three decades,
but its use as a quantitative method for measuring the relative expression of the target …

Stain-Free technology as a normalization tool in Western blot analysis

A Gürtler, N Kunz, M Gomolka, S Hornhardt… - Analytical …, 2013 - Elsevier
Western blots are used to specifically measure the relative quantities of proteins of interest in
complex biological samples. Quantitative measurements can be subject to error due to …

Coomassie staining as loading control in Western blot analysis

C Welinder, L Ekblad - Journal of proteome research, 2011 - ACS Publications
In Western blotting, immunodetection of housekeeping proteins is routinely performed to
detect differences in electrophoresis loading. The present work describes a much faster and …

Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolism

CP Najt, S Adhikari, TD Heden, W Cui, ER Gansemer… - Cell reports, 2023 - cell.com
Organelle interactions play a significant role in compartmentalizing metabolism and
signaling. Lipid droplets (LDs) interact with numerous organelles, including mitochondria …

[HTML][HTML] Consumption of whole eggs promotes greater stimulation of postexercise muscle protein synthesis than consumption of isonitrogenous amounts of egg whites …

S Van Vliet, EL Shy, S Abou Sawan, JW Beals… - The American journal of …, 2017 - Elsevier
Background Protein in the diet is commonly ingested from whole foods that contain various
macro-and micronutrients. However, the effect of consuming protein within its natural whole …