Cryptic genetic variation promotes rapid evolutionary adaptation in an RNA enzyme

EJ Hayden, E Ferrada, A Wagner - Nature, 2011 - nature.com
Cryptic variation is caused by the robustness of phenotypes to mutations. Cryptic variation
has no effect on phenotypes in a given genetic or environmental background, but it can have …

Reducing the impact of PCR-mediated recombination in molecular evolution and environmental studies using a new-generation high-fidelity DNA polymerase

DJG Lahr, LA Katz - Biotechniques, 2009 - Taylor & Francis
PCR-mediated recombination can greatly impact estimates of diversity, both in
environmental studies and in analyses of gene family evolution. Here we measure chimera …

Modern methods for laboratory diversification of biomolecules

S Bratulic, AH Badran - Current opinion in chemical biology, 2017 - Elsevier
Highlights•We review diversification methods for directed evolution and genome
engineering.•Recent diversification approaches allow non-biased exploration of sequence …

An abundant evolutionarily conserved CSB-PiggyBac fusion protein expressed in Cockayne syndrome

JC Newman, AD Bailey, HY Fan, T Pavelitz… - PLoS …, 2008 - journals.plos.org
Cockayne syndrome (CS) is a devastating progeria most often caused by mutations in the
CSB gene encoding a SWI/SNF family chromatin remodeling protein. Although all CSB …

Go east: phylogeographies of Mauremys caspica and M. rivulata– discordance of morphology, mitochondrial and nuclear genomic markers and rare hybridization

U Fritz, D Ayaz, J Buschbom, HG Kami… - Journal of …, 2008 - academic.oup.com
In recent years many cases of hybridization and introgression became known for
chelonians, requiring a better understanding of their speciation mechanisms …

From formamide to RNA, the path is tenuous but continuous

S Pino, JE Sponer, G Costanzo, R Saladino… - Life, 2015 - mdpi.com
Reactions of formamide (NH2COH) in the presence of catalysts of both terrestrial and
meteoritic origin yield, in plausible and variegated conditions, a large panel of precursors of …

iMAP: an integrated bioinformatics and visualization pipeline for microbiome data analysis

TM Buza, T Tonui, F Stomeo, C Tiambo, R Katani… - BMC …, 2019 - Springer
Background One of the major challenges facing investigators in the microbiome field is
turning large numbers of reads generated by next-generation sequencing (NGS) platforms …

Protocol for nearly full-length sequencing of HIV-1 RNA from plasma

Y Nadai, LM Eyzaguirre, NT Constantine, AM Sill… - PloS one, 2008 - journals.plos.org
Nearly full-length genome sequencing of HIV-1 using peripheral blood mononuclear cells
(PBMC) DNA as a template for PCR is now a relatively routine laboratory procedure …

Persistent infection and promiscuous recombination of multiple genotypes of an RNA virus within a single host generate extensive diversity

Z Weng, R Barthelson, S Gowda, ME Hilf… - PLoS …, 2007 - journals.plos.org
Recombination and reassortment of viral genomes are major processes contributing to the
creation of new, emerging viruses. These processes are especially significant in long-term …

Efficient silencing of reporter transgenes coupled to known functional promoters in sugarcane, a highly polyploid crop species

SR Mudge, K Osabe, RE Casu, GD Bonnett… - Planta, 2009 - Springer
Sugarcane is a crop of great interest for engineering of sustainable biomaterials and biofuel
production. Isolated sugarcane promoters have generally not maintained the expected …