Antifreeze proteins: Novel applications and navigation towards their clinical application in cryobanking

MD Ekpo, J Xie, Y Hu, X Liu, F Liu, J Xiang… - International Journal of …, 2022 - mdpi.com
Antifreeze proteins (AFPs) or thermal hysteresis (TH) proteins are biomolecular gifts of
nature to sustain life in extremely cold environments. This family of peptides, glycopeptides …

[HTML][HTML] Antifreeze proteins for low-temperature preservation in reproductive medicine: a systematic review over the last three decades

LFL Correia, BRC Alves, RITP Batista, P Mermillod… - Theriogenology, 2021 - Elsevier
Antifreeze proteins (AFPs) are synthesized by diverse non-mammalian species, allowing
them to survive in severely cold environments. Since the 1990s, the scientific literature …

Potential role of ice-binding protein in mitochondria-lipid and ATP mechanisms during freezing of plant callus

E Kim, GS Kwon, S Choi, SY Kim, KY Heo… - Plant Physiology and …, 2024 - Elsevier
Plant calli, a perpetually undifferentiated cell culture, have defects in maintaining their
genetic fidelity during prolonged tissue culture. Cryopreservation using ice-binding proteins …

N-acetyl-L-cysteine improves the developmental competence of bovine oocytes and embryos cultured in vitro by attenuating oxidative damage and apoptosis

WS Sun, H Jang, MR Park, KB Oh, H Lee, S Hwang… - Antioxidants, 2021 - mdpi.com
Oxidative stress has been suggested to negatively affect oocyte and embryo quality and
developmental competence, resulting in failure to reach full term. In this study, we …

Application of nanoparticles and melatonin for cryopreservation of gametes and embryos

HW Choi, H Jang - Current Issues in Molecular Biology, 2022 - mdpi.com
Cryopreservation of gametes and embryos, a technique widely applied in human infertility
clinics and to preserve desirable genetic traits of livestock, has been developed over 30 …

Effects of Porcine Immature Oocyte Vitrification on Actin Microfilament Distribution and Chromatin Integrity During Early Embryo Development in vitro

A López, Y Ducolomb, E Casas… - Frontiers in Cell and …, 2021 - frontiersin.org
Vitrification is mainly used to cryopreserve female gametes. This technique allows
maintaining cell viability, functionality, and developmental potential at low temperatures into …

Extended Temperature Range of the Ice-Binding Protein Activity

V Sirotinskaya, M Bar Dolev, V Yashunsky, L Bahari… - Langmuir, 2024 - ACS Publications
Ice-binding proteins (IBPs) are expressed in various organisms for several functions, such
as protecting them from freezing and freeze injuries. Via adsorption on ice surfaces, IBPs …

The effect of ice-binding protein from Leucosporidium sp. AY30 (LeIBP) on physicochemical quality and microstructure of largemouth bass during frozen storage

J Ren, F Hu, M Meenu, L Hu, Y Mao, T Song… - Innovative Food Science …, 2025 - Elsevier
In this paper, the effect of different concentrations (0.01%, 0.05%, 0.1%, 0.2%, 0.5%) of ice-
binding protein from Leucosporidium sp. AY30 (LeIBP) on the microstructure and …

Effects of antifreeze protein from Lolium perenne L.(LpAFP) in the vitrification of in vitro-produced bovine embryos

RAS Júnior, R Desenzi, MMS Ramires, AF Souza… - Zygote, 2023 - cambridge.org
In the present study, the cryoprotective effects of Lolium perenne antifreeze protein (LpAFP)
on the vitrification of bovine embryos were evaluated. In vitro-produced blastocysts were …

Effect of antifreeze protein I in the freezing solution on in vivo-derived sheep embryos

LFL Correia, GR Leal, FZ Brandão, RITP Batista… - Research in Veterinary …, 2024 - Elsevier
This study evaluated the effects of different antifreeze protein type I (AFP I) concentrations
added to a slow freezing solution in sheep in vivo-derived embryos. Good-quality embryos …