This review aims to assess and recommend approaches for targeted and agnostic High
Throughput Sequencing of RNA viruses in a variety of sample matrices. HTS also referred to …

Abstract Cell-associated (CA) HIV RNA has received much attention in recent years as a
surrogate measure of the efficiency of HIV latency reversion and because it may provide an …

The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the
evident requirement for quantitative data analysis in molecular medicine, biotechnology …

Cell-associated (CA) HIV-1 RNA is considered a potential marker for assessment of viral
reservoir dynamics and antiretroviral therapy (ART) response in HIV-infected patients …

Compromised RNA quality is suggested to lead to unreliable results in gene expression
studies. Therefore, assessment of RNA integrity and purity is deemed essential prior to …

Large-scale sequencing projects have revealed an unexpected complexity in the origins,
structures and functions of mammalian transcripts. Many loci are known to produce …

The endometrium becomes receptive to the embryo only in the mid-luteal phase, but not in
the other stages of the menstrual cycle. Endometrial factors play an important role in …

The endogenous opioid system (EOS) plays a critical role in addictive processes. Molecular
dysregulations in this system may be specific for different stages of addiction cycle and …

Reverse transcription (RT) is a crucial step in most RNA analysis methods. Optimizing
protocols for this initial stage is critical for effective target detection, particularly when …

Comparative biology includes the comparison of transcriptome and quantitative real-time RT-
PCR (qRT-PCR) data sets in a range of species to detect evolutionarily conserved and …