Light-sheets and smart microscopy, an exciting future is dawning

S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Recent advances in oblique plane microscopy

J Kim - Nanophotonics, 2023 - degruyter.com
Oblique plane microscopy (OPM) directly captures object information in a plane tilted from
the focal plane of the objective lens without the need for slow z-stack acquisition. This …

Mesoscopic oblique plane microscopy via light-sheet mirroring

S Daetwyler, BJ Chang, B Chen, FF Voigt, D Rajendran… - Optica, 2023 - opg.optica.org
Understanding the intricate interplay and inter-connectivity of biological processes across an
entire organism is important in various fields of biology, including cardiovascular research …

Endogenous tagging of multiple cellular components in the sea anemone Nematostella vectensis

A Paix, S Basu, P Steenbergen… - Proceedings of the …, 2023 - National Acad Sciences
The cnidarian Nematostella vectensis has developed into a powerful model system to study
the mechanisms underlying animal development, regeneration, and evolution. However …

Mesoscopic oblique plane microscopy with a diffractive light-sheet for large-scale 4D cellular resolution imaging

W Shao, M Chang, K Emmerich, PO Kanold, JS Mumm… - Optica, 2022 - opg.optica.org
Fundamental understanding of large-scale dynamic connectivity within a living organism
requires volumetric imaging over a large field of view (FOV) at biologically relevant speed …

Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging

S Park, CH Min, E Choi, JS Choi, K Park, S Han… - Scientific Reports, 2025 - nature.com
Human cerebral organoids serve as a quintessential model for deciphering the complexities
of brain development in a three-dimensional milieu. However, imaging these organoids …

Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics

S Cheng, Y Nakatani, G Gagliano, N Saliba… - npj Imaging, 2024 - nature.com
Single-molecule localization microscopy has revealed cellular architectures and molecular
dynamics beyond the diffraction limit of light. However, imaging thick samples presents …

Mesoscopic axially swept oblique plane microscope for the imaging of freely moving organisms with near-isotropic resolution

S Davis, JR Sommernes, S Hambura… - Biomedical Optics …, 2024 - opg.optica.org
Rapid three-dimensional imaging over extended fields of view (FOVs) is crucial to the study
of organism-wide systems and biological processes in vivo. Selective-plane illumination …

A mesoscopic axially swept oblique plane microscope for imaging of freely moving organisms with near-isotropic resolution

R Prevedel, S Davis, JR Sommernes, L Riedel, A Gil… - 2024 - munin.uit.no
Rapid three-dimensional imaging over extended fields of views (FOVs) is crucial to the study
of organism-wide systems and biological processes in vivo. Selective-plane illumination …