RISC assembly: Coordination between small RNAs and Argonaute proteins

H Kobayashi, Y Tomari - Biochimica et Biophysica Acta (BBA)-Gene …, 2016 - Elsevier
Non-coding RNAs generally form ribonucleoprotein (RNP) complexes with their partner
proteins to exert their functions. Small RNAs, including microRNAs, small interfering RNAs …

Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?

K Nakanishi - Wiley Interdisciplinary Reviews: RNA, 2016 - Wiley Online Library
RNA silencing is a eukaryote‐specific phenomenon in which microRNAs and small
interfering RNAs degrade messenger RNAs containing a complementary sequence. To this …

Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes

IM Schopp, CC Amaya Ramirez, J Debeljak… - Nature …, 2017 - nature.com
Understanding the function of the thousands of cellular proteins is a central question in
molecular cell biology. As proteins are typically part of multiple dynamic and often …

Whats, hows and whys of programmed DNA elimination in Tetrahymena

T Noto, K Mochizuki - Open biology, 2017 - royalsocietypublishing.org
Programmed genome rearrangements in ciliates provide fascinating examples of flexible
epigenetic genome regulations and important insights into the interaction between …

AGO1 and HSP90 buffer different genetic variants in Arabidopsis thaliana

T Lemus, GA Mason, KL Bubb, CM Alexandre… - Genetics, 2023 - academic.oup.com
Abstract Argonaute 1 (AGO1), the principal protein component of microRNA-mediated
regulation, plays a key role in plant growth and development. AGO1 physically interacts with …

Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila

T Cheng, Y Wang, J Huang, X Chen, X Zhao… - Marine Life Science & …, 2019 - Springer
Epigenetic research focuses on heritable changes beyond the DNA sequence, which has
led to a revolution in biological studies and benefits in many other fields. The well-known …

Small-RNA-mediated genome-wide trans-recognition network in Tetrahymena DNA elimination

T Noto, K Kataoka, JH Suhren, A Hayashi, KJ Woolcock… - Molecular cell, 2015 - cell.com
Small RNAs are used to silence transposable elements (TEs) in many eukaryotes, which
use diverse evolutionary solutions to identify TEs. In ciliated protozoans, small-RNA …

Functional analysis of the methyltransferase SMYD in the single-cell model organism Tetrahymena thermophila

X Zhao, Y Li, L Duan, X Chen, F Mao, M Juma… - Marine Life Science & …, 2020 - Springer
Lysine methylation of histones and non-histones plays a pivotal role in diverse cellular
processes. The SMYD (SET and MYND domain) family methyltransferases can methylate …

Phosphorylation of an HP1-like protein regulates heterochromatin body assembly for DNA elimination

K Kataoka, K Mochizuki - Developmental cell, 2015 - cell.com
Heterochromatic loci are often assembled into higher-order heterochromatin bodies in
diverse eukaryotes. However, the formation and biological roles of heterochromatin bodies …

Targeted Gene Disruption by Ectopic Induction of DNA Elimination in Tetrahymena

A Hayashi, K Mochizuki - Genetics, 2015 - academic.oup.com
Tetrahymena is a useful eukaryotic model for biochemistry and molecular cell biology
studies. We previously demonstrated that targeted ectopic DNA elimination, also called co …