The reprogramming of somatic cells with defined factors, which converts cells from one
lineage into cells of another, has greatly reshaped our traditional views on cell identity and …

Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

Single-cell gene expression studies promise to reveal rare cell types and cryptic states, but
the high variability of single-cell RNA-seq measurements frustrates efforts to assay …

Aging is the major risk factor for many human diseases. In vitro studies have demonstrated
that cellular reprogramming to pluripotency reverses cellular age, but alteration of the aging …

The past 10 years have seen great advances in our ability to manipulate cell fate, including
the induction of pluripotency in vitro to generate induced pluripotent stem cells (iPSCs). This …

The discovery of somatic cell nuclear transfer proved that somatic cells can carry the same
genetic code as the zygote, and that activating parts of this code are sufficient to reprogram …

Direct lineage reprogramming involves the conversion of cellular identity. Single-cell
technologies are useful for deconstructing the considerable heterogeneity that emerges …

Summary Oct4, Sox2, Klf4, and cMyc (OSKM) reprogram somatic cells to pluripotency. To
gain a mechanistic understanding of their function, we mapped OSKM-binding, stage …

A revolution in cellular measurement technology is under way: For the first time, we have the
ability to monitor global gene regulation in thousands of individual cells in a single …

Defining the transcriptional dynamics of a temporal process such as cell differentiation is
challenging owing to the high variability in gene expression between individual cells. Time …