The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Organic fluorophores are indispensable tools in cells, tissue and in vivo imaging, and have
enabled much progress in the wide range of biological and biomedical fields. However …

The quality and application of super-resolution fluorescence imaging greatly lie in the dyes'
properties, including photostability, brightness, and Stokes shift. Here we report a synergistic …

Fluorescence microscopy relies on dyes that absorb and then emit photons. In addition to
fluorescence, fluorophores can undergo photochemical processes that decrease quantum …

Rhodamine dyes exist in equilibrium between a fluorescent zwitterion and a nonfluorescent
lactone. Tuning this equilibrium toward the nonfluorescent lactone form can improve cell …

Insufficient brightness of fluorophores poses a major bottleneck for the advancement of
super-resolution microscopes. Despite being widely used, many rhodamine dyes exhibit sub …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Live-cell single-molecule localization microscopy has advanced with the development of self-
blinking rhodamines. A p K cycling of< 6 is recognized as the criterion for self-blinking, yet a …

Visualizing and manipulating the behavior of proteins is crucial to understanding the
physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain …

The light-promoted conversion of extensively used cyanine dyes to blue-shifted emissive
products has been observed in various contexts. However, both the underlying mechanism …