Decorating microbes: surface display of proteins on Escherichia coli

E van Bloois, RT Winter, H Kolmar, MW Fraaije - Trends in biotechnology, 2011 - cell.com
Bacterial surface display entails the presentation of recombinant proteins or peptides on the
surface of bacterial cells. Escherichia coli is the most frequently used bacterial host for …

Bacterial whole-cell biocatalysts by surface display of enzymes: toward industrial application

J Schüürmann, P Quehl, G Festel, J Jose - Applied microbiology and …, 2014 - Springer
Despite the first report on the bacterial display of a recombinant peptide appeared almost 30
years ago, industrial application of cells with surface-displayed enzymes is still limited. To …

The autodisplay story, from discovery to biotechnical and biomedical applications

J Jose, TF Meyer - Microbiology and Molecular Biology Reviews, 2007 - Am Soc Microbiol
Among the pathways used by gram-negative bacteria for protein secretion, the
autotransporter pathway represents a solution of impressive simplicity. Proteins are …

E. coli surface display of streptavidin for directed evolution of an allylic deallylase

T Heinisch, F Schwizer, B Garabedian, E Csibra… - Chemical …, 2018 - pubs.rsc.org
Artificial metalloenzymes (ArMs hereafter) combine attractive features of both homogeneous
catalysts and enzymes and offer the potential to implement new-to-nature reactions in living …

Development of a first-in-class small-molecule inhibitor of the C-terminal Hsp90 dimerization

S Bhatia, L Spanier, D Bickel, N Dienstbier… - ACS central …, 2022 - ACS Publications
Heat shock proteins 90 (Hsp90) are promising therapeutic targets due to their involvement in
stabilizing several aberrantly expressed oncoproteins. In cancerous cells, Hsp90 expression …

Highly crystalline post‐consumer PET waste hydrolysis by surface displayed PETase using a bacterial whole‐cell biocatalyst

D Gercke, C Furtmann, IEP Tozakidis, J Jose - ChemCatChem, 2021 - Wiley Online Library
The enzymatic degradation of polyethylene terephthalate (PET) is a promising new
approach for the environmentally friendly recycling of PET waste, but so far, low degradation …

Autodisplay: efficient bacterial surface display of recombinant proteins

J Jose - Applied microbiology and biotechnology, 2006 - Springer
To display a protein or peptide with a distinct function at the surface of a living bacterial cell
is a challenging exercise with constantly increasing impact in many areas of biochemistry …

Engineering novel and improved biocatalysts by cell surface display

MR Smith, E Khera, F Wen - Industrial & engineering chemistry …, 2015 - ACS Publications
Biocatalysts, especially enzymes, have the ability to catalyze reactions with high product
selectivity, utilize a broad range of substrates, and maintain activity at low temperature and …

Display of active enzymes on the cell surface of Escherichia coli using PgsA anchor protein and their application to bioconversion

J Narita, K Okano, T Tateno, T Tanino, T Sewaki… - Applied microbiology …, 2006 - Springer
We have developed a novel Escherichia coli cell surface display system by employing PgsA
as an anchoring motif. In our display system, C-terminal fusion to PgsA anchor protein from …

Autotransporter-based cell surface display in Gram-negative bacteria

T Nicolay, J Vanderleyden… - Critical reviews in …, 2015 - Taylor & Francis
Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …