Guanine-rich DNA sequences can fold into stable four-stranded structures called G-
quadruplexes or G4s. Research in the past decade demonstrated that G4 structures are …

DNA double-strand break (DSB) repair by homologous recombination is initiated by DNA
end resection, a process involving the controlled degradation of the 5′-terminated strands …

Pif1 family helicases are multifunctional proteins conserved in eukaryotes, from yeast to
humans. They are important for the genome maintenance in both nuclei and mitochondria …

DNA double-strand break (DSB) repair is initiated by DNA end resection. CtIP acts in short-
range resection to stimulate MRE11–RAD50–NBS1 (MRN) to endonucleolytically cleave …

HROB promotes the MCM8-9 helicase in DNA damage response. To understand how
HROB activates MCM8-9, we defined their interaction interface. We showed that HROB …

Replication protein A (RPA) binds single-stranded DNA (ssDNA) and serves critical
functions in eukaryotic DNA replication, the DNA damage response, and DNA repair. During …

Single-stranded DNA (ssDNA) intermediates which emerge during DNA metabolic
processes are shielded by replication protein A (RPA). RPA binds to ssDNA and acts as a …

Homologous recombination enables cells to overcome the threat of DNA double-strand
breaks (DSBs), allowing for repair without the loss of genetic information. Central to the …

Here, we employed a fluorescence-based single molecule method called nuclease-induced
stepwise photodropping (NISP) to measure in real time the DNA degradation mediated by …

During the repair of DNA double-strand breaks (DSBs), de novo synthesized DNA strands
can displace the parental strand to generate single-strand DNAs (ssDNAs). Many …