To initiate DNA replication, it is essential to properly assemble a pair of replicative helicases
at each replication origin. While the general principle of this process applies universally from …

The paradigm of cellular systems as deterministic machines has long guided our
understanding of biology. Advancements in technology and methodology, however, have …

The eukaryotic replisome is assembled around the CMG (CDC45-MCM-GINS) replicative
helicase, which encircles the leading-strand DNA template at replication forks. When CMG …

Human RECQL4, a member of the RecQ helicase family, plays a role in maintaining
genomic stability, but its precise function remains unclear. The N-terminus of RECQL4 has …

FANCM is a multifunctional DNA repair enzyme that acts as a sensor and coordinator of
replication stress responses, especially interstrand crosslink (ICL) repair mediated by the …

Activation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for
replication origin firing, and as such for faithful genetic inheritance. Our biochemical and …

Accurate and complete replication of genetic information is a fundamental process of every
cell division. The replication licensing is the first essential step that lays the foundation for …

To prevent detrimental chromosome re-replication, DNA loading of a double hexamer of the
minichromosome maintenance (MCM) replicative helicase is temporally separated from …

Energy metabolism is a fundamental aspect of the aggressiveness and invasiveness of
breast cancer (BC), the neoplasm that most affects women worldwide. Nonetheless, the …

Metazoan genomes are copied bidirectionally from thousands of replication origins.
Replication initiation entails the assembly and activation of two CMG helicases (Cdc45⋅ …