Repeated DNA makes up a large fraction of a typical mammalian genome, and some
repetitive elements are able to move within the genome (transposons and retrotransposons) …

Increasing evidence reveals that random insertion of gene transfer vectors into the genome
of repopulating hematopoietic cells may alter their fate in vivo. Although most insertional …

For decades, identifying the regions of a bacterial chromosome that are necessary for
viability has relied on mapping integration sites in libraries of random transposon mutants to …

Long-range regulatory interactions play an important role in shaping gene-expression
programs. However, the genomic features that organize these activities are still poorly …

Abstract The Sleeping Beauty (SB) transposon is a promising technology platform for gene
transfer in vertebrates; however, its efficiency of gene insertion can be a bottleneck in …

Transposons are mobile genetic elements that can be used to integrate transgenes into host
cell genomes. The piggyBac transposon system has been used for transgenesis of insects …

Transposable elements are DNA segments with the unique ability to move about in the
genome. This inherent feature can be exploited to harness these elements as gene vectors …

Genomic studies in the mouse have been slowed by the lack of transposon-mediated
mutagenesis. However, since the resurrection of Sleeping Beauty (SB), the possibility of …

Viruses and transposons are efficient tools for permanently delivering foreign DNA into
vertebrate genomes but exhibit diminished activity when cargo exceeds 8 kilobases (kb) …

Abstract The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene
discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions …