Messenger RNA expression patterns in bovine embryos derived from in vitro procedures and their implications for development

C Wrenzycki, D Herrmann, A Lucas-Hahn… - Reproduction …, 2004 - CSIRO Publishing
The preimplantation bovine embryo is initially under the control of maternal genomic
information that is accumulated during oogenesis. The genetic programme of development …

Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos

K Goossens, M Van Poucke, A Van Soom… - BMC developmental …, 2005 - Springer
Background Real-time quantitative PCR is a sensitive and very efficient technique to
examine gene transcription patterns in preimplantation embryos, in order to gain information …

Temporal divergence in the pattern of messenger RNA expression in bovine embryos cultured from the zygote to blastocyst stage in vitro or in vivo

P Lonergan, D Rizos, A Gutiérrez-Adán… - Biology of …, 2003 - academic.oup.com
The objective of this study was to examine the time during the postfertilization period that
gene expression patterns in in vitro-cultured bovine embryos diverge from those of their in …

Osmotic stress and cryoinjury of koala sperm: an integrative study of the plasma membrane, chromatin stability and mitochondrial function

SD Johnston, N Satake, Y Zee… - …, 2012 - rep.bioscientifica.com
This study investigated whether cryopreservation-induced injury to koala spermatozoa could
be explained using an experimental model that mimics the structural and physiological …

Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro

S Mamo, AB Gal, S Bodo, A Dinnyes - BMC developmental biology, 2007 - Springer
Background Real-time PCR is an efficient tool to measure transcripts and provide valuable
quantitative information on gene expression of preimplantation stage embryos. Finding valid …

Epigenetic differences between male and female bovine blastocysts produced in vitro

P Bermejo-Alvarez, D Rizos, D Rath… - Physiological …, 2008 - journals.physiology.org
Epigenetic differences between male and female bovine blastocysts provide a plausible link
between physiological and gene transcription differences observed between male and …

Molecular control of mitochondrial function in preimplantation mouse embryos

J Thundathil, F Filion, LC Smith - Molecular reproduction and …, 2005 - Wiley Online Library
Mitochondria play a key role in a number of physiological events during all stages of life,
including the very first stages following fertilization. It is, therefore, important to understand …

[HTML][HTML] Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues

EL Rebouças, JJN Costa, MJ Passos… - Brazilian Archives of …, 2013 - SciELO Brasil
The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a
technique for mRNA expression analysis in different tissues. Real-time PCR is widely used …

[PDF][PDF] Seasonal effects on gene expression, cleavage timing, and developmental competence of bovine preimplantation embryos

M Gendelman, A Aroyo, S Yavin, Z Roth - Reproduction, 2010 - academia.edu
We examined the association between season and expression of genes involved in early
embryonic development with an emphasis on cleavage rate and timing of the first embryonic …

Increase of mitochondrial DNA content and transcripts in early bovine embryogenesis associated with upregulation of mtTFA and NRF1 transcription factors

P May-Panloup, X Vignon, MF Chrétien… - Reproductive Biology …, 2005 - Springer
Background Recent work has shown that mitochondrial biogenesis and mitochondrial
functions are critical determinants of embryonic development. However, the expression of …