Since the serendipitous discovery 20 years ago of bona fide camelid heavy-chain
antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies …

Nanobodies can be seen as next‐generation tools for the recognition and modulation of
antigens that are inaccessible to conventional antibodies. Due to their compact structure and …

Antibodies are key reagents to investigate cellular processes. The development of
recombinant antibodies and binders derived from natural protein scaffolds has expanded …

Owing to peculiar properties of nanobody, including nanoscale size, robust structure, stable
and soluble behaviors in aqueous solution, reversible refolding, high affinity and specificity …

Single-domain antibodies (sdAbs) have substantially expanded the possibilities of
advanced cellular imaging such as live-cell or super-resolution microscopy to visualize …

Detection of intracerebral targets with imaging probes is challenging due to the non-
permissive nature of blood-brain barrier (BBB). The present work describes two novel single …

Understanding how building blocks of life contribute to physiology is greatly aided by protein
identification and cellular localization. The two main labeling approaches developed over …

Chromobodies are intracellular nanoprobes that combine the specificity of antibodies with
the convenience of live fluorescence imaging in a flexible, DNA-encoded reagent. Here, we …

Vimentin has become an important biomarker for epithelial-mesenchymal transition (EMT), a
highly dynamic cellular process involved in the initiation of metastasis and cancer …

In biomedical research there is an ongoing demand for new technologies, which help to
elucidate disease mechanisms and provide the basis to develop novel therapeutics. In this …