Production of biopharmaceuticals in E. coli: current scenario and future perspectives

MN Baeshen, AM Al-Hejin, RS Bora… - … of microbiology and …, 2015 - koreascience.kr
Escherichia coli is the most preferred microorganism to express heterologous proteins for
therapeutic use, as around 30% of the approved therapeutic proteins are currently being …

[HTML][HTML] Production and purification of the multifunctional enzyme horseradish peroxidase

O Spadiut, C Herwig - Pharmaceutical bioprocessing, 2013 - ncbi.nlm.nih.gov
The oxidoreductase horseradish peroxidase (HRP) is used in numerous industrial and
medical applications. In this review, we briefly describe this well-studied enzyme and focus …

[HTML][HTML] Influence of camel milk on the hepatitis C virus burden of infected patients

EM El-Fakharany, NA El-Baky… - Experimental and …, 2017 - spandidos-publications.com
Hepatitis C virus (HCV) infection represents a world health problem and no protective
vaccine or effective drug currently exists. For economic reasons, many patients use …

[HTML][HTML] Screening the anti infectivity potentials of native N-and C-lobes derived from the camel lactoferrin against hepatitis C virus

EM Redwan, EM El-Fakharany, VN Uversky… - … and alternative medicine, 2014 - Springer
Abstract Background Hepatitis C virus (HCV) infection represents a worldwide health threat
that still needs efficient protective vaccine and/or effective drug. The traditional medicine …

[HTML][HTML] Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7. 5 hepatoma

EM El-Fakharany, N Abedelbaky, BM Haroun… - Virology journal, 2012 - Springer
Purpose To extend the study of the camel milk proteins which have antiviral activity against
HCV, camel naïve polyclonal IgGs, α-lactalbumin were purified from camel milk and their …

Therapeutic alpha-interferons protein: structure, production, and biosimilar

NA El-Baky, EM Redwan - Preparative Biochemistry and …, 2015 - Taylor & Francis
In 2007, the world solemnized the golden jubilee of the discovery of interferon (IFN).
Interferon is a small protein messenger called a pluripotent cytokine, produced by several …

Expression of soluble and active interferon consensus in SUMO fusion expression system in E. coli

K Peciak, R Tommasi, J Choi, S Brocchini… - Protein expression and …, 2014 - Elsevier
Protein production can be improved if methods for soluble protein expression are
developed. Interferon consensus (IFN-con) is used to treat hepatitis C. IFN-con has superior …

[HTML][HTML] A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha

NA El-Baky, EM El-Fakharany, SA Sabry, ER El-Helow… - Biology, 2022 - mdpi.com
Simple Summary As a result of increasing demand for the pleiotropic cytokine TNF-α,
recombinant human TNF-α protein with appropriate bioactivities was produced in several …

Simple procedure applying lactose induction and one‐step purification for high‐yield production of rhCIFN

H Bashir, N Ahmed, MA Khan, AU Zafar… - Biotechnology and …, 2016 - Wiley Online Library
Recombinant consensus interferon (CIFN) is a therapeutic protein with molecular weight of
19.5 kDa having broad spectrum antiviral activity. Recombinant human CIFN (rhCIFN) has …

[HTML][HTML] De novo expression and antibacterial potential of four lactoferricin peptides in cell-free protein synthesis system

N Abd El-Baky, MA Elkhawaga, ES Abdelkhalek… - Biotechnology …, 2021 - Elsevier
For the first time, we produced four lactoferricin (LFcin) peptides by a cell-free (in vitro)
method. These short antimicrobial peptides were expressed in an E. coli cell-free protein …