Challenges in recombinant brain-derived neurotrophic factor production
Brain-derived neurotrophic factor (BDNF) is a neurotrophin of marked commercial, scientific,
diagnostic, and therapeutic interest. The preservation of its structural cystine-knot is the main …
diagnostic, and therapeutic interest. The preservation of its structural cystine-knot is the main …
Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves
Despite the central role that antibodies play in the adaptive immune system and in
biotechnology, much remains unknown about the quantitative relationship between an …
biotechnology, much remains unknown about the quantitative relationship between an …
Nanoformulation of brain‐derived neurotrophic factor with target receptor‐triggered‐release in the central nervous system
Brain‐derived neurotrophic factor (BDNF) is identified as a potent neuroprotective and
neuroregenerative agent for many neurological diseases. Regrettably, its delivery to the …
neuroregenerative agent for many neurological diseases. Regrettably, its delivery to the …
Yeast surface display: new opportunities for a time-tested protein engineering system
M Raeeszadeh-Sarmazdeh, ET Boder - Yeast surface display, 2022 - Springer
Yeast surface display has proven to be a powerful tool for the discovery of antibodies and
other novel binding proteins and for engineering the affinity and selectivity of existing …
other novel binding proteins and for engineering the affinity and selectivity of existing …
Structural insights into VLR fine specificity for blood group carbohydrates
BC Collins, RJ Gunn, TR McKitrick, RD Cummings… - Structure, 2017 - cell.com
High-quality reagents to study and detect glycans with high specificity for research and
clinical applications are severely lacking. Here, we structurally and functionally characterize …
clinical applications are severely lacking. Here, we structurally and functionally characterize …
Identification of variable lymphocyte receptors that can target therapeutics to pathologically exposed brain extracellular matrix
BJ Umlauf, PA Clark, JM Lajoie, JV Georgieva… - Science …, 2019 - science.org
Diseases that lead to blood-brain barrier (BBB) disruption will pathologically expose
normally inaccessible brain extracellular matrix (ECM) to circulating blood components …
normally inaccessible brain extracellular matrix (ECM) to circulating blood components …
Yeast display biopanning identifies human antibodies targeting glioblastoma stem-like cells
Glioblastoma stem-like cells (GSC) are hypothesized to evade current therapies and cause
tumor recurrence, contributing to poor patient survival. Existing cell surface markers for GSC …
tumor recurrence, contributing to poor patient survival. Existing cell surface markers for GSC …
One-pot synthesis of cellobiose from sucrose using sucrose phosphorylase and cellobiose phosphorylase co-displaying Pichia pastoris as a reusable whole-cell …
Cellobiose has received increasing attention in various industrial sectors, ranging from food
and feed to cosmetics. The development of large-scale cellobiose applications requires a …
and feed to cosmetics. The development of large-scale cellobiose applications requires a …
Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein
P Heinzelman, PA Romero - PLoS One, 2021 - journals.plos.org
Understanding how human ACE2 genetic variants differ in their recognition by SARS-CoV-2
can facilitate the leveraging of ACE2 as an axis for treating and preventing COVID-19. In this …
can facilitate the leveraging of ACE2 as an axis for treating and preventing COVID-19. In this …
Determination of binding affinity upon mutation for type I dockerin–cohesin complexes from Clostridium thermocellum and Clostridium cellulolyticum using deep …
CA Kowalsky, TA Whitehead - Proteins: Structure, Function, and …, 2016 - Wiley Online Library
The comprehensive sequence determinants of binding affinity for type I cohesin toward
dockerin from Clostridium thermocellum and Clostridium cellulolyticum was evaluated using …
dockerin from Clostridium thermocellum and Clostridium cellulolyticum was evaluated using …