[HTML][HTML] Steady-state fluorescence quenching applications for studying protein structure and dynamics
Fluorescence quenching methods are useful to obtain information about the conformational
and/or dynamic changes of proteins in complex macromolecular systems. In this review …
and/or dynamic changes of proteins in complex macromolecular systems. In this review …
Mechanism of the calcium-regulation of muscle contraction—In pursuit of its structural basis—
T Wakabayashi - Proceedings of the Japan Academy, Series B, 2015 - jstage.jst.go.jp
The author reviewed the research that led to establish the structural basis for the mechanism
of the calcium-regulation of the contraction of striated muscles. The target of calcium ions is …
of the calcium-regulation of the contraction of striated muscles. The target of calcium ions is …
Clinically divergent mutation effects on the structure and function of the human cardiac tropomyosin overlap
M McConnell, L Tal Grinspan, MR Williams… - Biochemistry, 2017 - ACS Publications
The progression of genetically inherited cardiomyopathies from an altered protein structure
to clinical presentation of disease is not well understood. One of the main roadblocks to …
to clinical presentation of disease is not well understood. One of the main roadblocks to …
4.13 thin filament regulation
MA Geeves - Comprehensive Biophysics, 2012 - kar.kent.ac.uk
The review summarizes the current state of knowledge of the calcium regulation of striated
muscle contraction via the thin filament proteins, tropomyosin and troponin. The description …
muscle contraction via the thin filament proteins, tropomyosin and troponin. The description …
In situ time-resolved FRET reveals effects of sarcomere length on cardiac thin-filament activation
During cardiac thin-filament activation, the N-domain of cardiac troponin C (N-cTnC) binds to
Ca 2+ and interacts with the actomyosin inhibitory troponin I (cTnI). The interaction between …
Ca 2+ and interacts with the actomyosin inhibitory troponin I (cTnI). The interaction between …
Calcium structural transition of human cardiac troponin C in reconstituted muscle fibres as studied by site-directed spin labelling
M Nakamura, S Ueki, H Hara, T Arata - Journal of molecular biology, 2005 - Elsevier
The in situ structure of human cardiac troponin C (hcTnC) has been studied with site-
directed, spin labelling, electron paramagnetic resonance (SDSL-EPR). Analysis of the in …
directed, spin labelling, electron paramagnetic resonance (SDSL-EPR). Analysis of the in …
Tropomyosin and troponin cooperativity on the thin filament
SE Boussouf, MA Geeves - Regulatory Mechanisms of Striated Muscle …, 2007 - Springer
The regulation of muscle contraction by the thin filament proteins tropomyosin (Tm) and
troponin (Tn) has remained an area of interest since the proteins were first discovered 40 …
troponin (Tn) has remained an area of interest since the proteins were first discovered 40 …
Myopathy-causing Q147P TPM2 mutation shifts tropomyosin strands further towards the open position and increases the proportion of strong-binding cross-bridges …
OE Karpicheva, AO Simonyan, NV Kuleva… - … et Biophysica Acta (BBA …, 2016 - Elsevier
The molecular mechanisms of skeletal muscle dysfunction in congenital myopathies remain
unclear. The present study examines the effect of a myopathy-causing mutation Q147P in β …
unclear. The present study examines the effect of a myopathy-causing mutation Q147P in β …
Dynamics of tropomyosin in muscle fibers as monitored by saturation transfer EPR of bi-functional probe
The dynamics of four regions of tropomyosin was assessed using saturation transfer
electron paramagnetic resonance in the muscle fiber. In order to fully immobilize the spin …
electron paramagnetic resonance in the muscle fiber. In order to fully immobilize the spin …
Fluorescence resonance energy transfer between residues on troponin and tropomyosin in the reconstituted thin filament: modeling the troponin–tropomyosin …
C Kimura-Sakiyama, Y Ueno, K Wakabayashi… - Journal of molecular …, 2008 - Elsevier
Troponin (Tn), in association with tropomyosin (Tm), plays a central role in the calcium
regulation of striated muscle contraction. Fluorescence resonance energy transfer (FRET) …
regulation of striated muscle contraction. Fluorescence resonance energy transfer (FRET) …