In most bioluminescence systems the oxidation of luciferin and production of light is
catalyzed by luciferases. Protein engineering studies have shown that thermostable proteins …

The bright bioluminescence catalyzed by Photinus pyralis firefly luciferase (Fluc) enables a
vast array of life science research such as bio imaging in live animals and sensitive in vitro …

Physical and mathematical methods such as fluorescence and circular dichroism
spectroscopies as well as MCR-ALS chemometric analysis and Wyman linkage theory were …

Multi-color bioluminescence is developed using the introduction of single/double disulfide
bridges in firefly luciferase. The bioluminescence reaction, which uses luciferin, Mg2+-ATP …

The effect of proline on refolding and unfolding kinetics as well as activity of lipase from
Pseudomonas fluorescens was determined using stopped-flow fluorescence and UV/Vis …

Lipases with high kinetic stability and enzymatic efficiency in the human gastro-intestinal
tract may help against exocrine pancreatic insufficiency. Here we mimic gastric conditions to …

Aims: The probability of establishing electrostatic interactions due to the abundance of
charged hydrophilic residues and especially arginine is considered the most important …

Possible roles of two different Arginine (Arg; R) 213 and 337 on kinetic and structural
stability of Photinus pyralis luciferase have been investigated using thermal and chemical …

We present here the effect of firefly luciferase surface charge saturation and the presence of
some additives on its thermal‐induced aggregation. Three mutants of firefly luciferase …

لیپازها به عنوان یک گروه آنزیمی مهم باعث هیدورلیز و سنتز استرها می‌شوند. لیپاز سودوموناس
فلوئورسانس یک نوع گرمادوست از لیپازها است که وزن مولکولی آن در حدود 33 کیلودالتون می‌باشد …‎