Fluorescent proteins have become an invaluable tool in cell biology. The green fluorescent
protein variant EGFP is especially widely applied. Use of fluorescent proteins, including …

Yellow fluorescent protein (YFP 10C) is widely used as a probe in biology, but its complex
photochemistry gives rise to unusual behavior that requires fuller definition. Here we …

Fluorescence resonance energy transfer (FRET) is an extremely effective tool to detect
molecular interaction at suboptical resolutions. One of the techniques for measuring FRET is …

After a brief overview of NMR and X-ray crystallography studies on protein flexibility under
pressure, we summarize the effects of hydrostatic pressure on the native fold of azurin from …

Over the past 25 years, single-molecule spectroscopy has developed into a widely used tool
in multiple disciplines of science. The diversity of routinely recorded emission spectra does …

Biological rotary motors are essential for life. For example, nucleic acids are processed and
modified by a variety of motors. But also in the lipid membranes surrounding different cell …

Protein-based material design provides great advantages to developing smart biomaterials
with tunable structures and desired functions. They have been widely used in many …

Photostability is one of the crucial properties of a fluorophore which strongly influences the
quality of single molecule-based super-resolution imaging. Enhanced yellow fluorescent …

X-ray diffraction analysis of pressure-induced structural changes in the Aequorea yellow
fluorescent protein Citrine reveals the structural basis for the continuous fluorescence peak …

Time-dependent fluctuations in the catalysis rate (δ k (t)) observed in single-enzyme
experiments were found in a particular study to have an autocorrelation function decaying …