We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extract RNA
from as little as 10,000 cells and to measure the expression levels of several target mRNAs …

The genus Edwardsiella comprises a genetically distinct taxon related to other members of
the family Enterobacteriaceae. It consists of bacteria differing strongly in their biochemical …

The biophysical properties of NMDA receptors are thought to be critical determinants
involved in the regulation of long‐term synaptic plasticity during neocortical development …

The rapid progress in the Human Genome Project has stimulated the development of
diverse analytical methods for mutation detection, elucidation of complex biological …

Accurate quantification of nucleic acids by competitive (RT)–PCR requires a valid internal
standard, a reference for data normalization and an adequate mathematical model for data …

One novel approach for the biological delivery of peptide drugs is to incorporate the
sequence of the peptide into the structure of a natural transport protein, such as human …

The cytochrome P450 aromatase (aromP450) deficient mice are infertile due to an
impairment of spermatogenesis associated with a decrease in sperm motility and inability to …

根据已报道的猪抗菌肽基因和看家基因茁-肌动蛋白() 基因序列, 分别设计和的引物, 探讨了PCR
体系中适宜的MgCl2 浓度, 循环次数, 以及2 对引物间竞争情况. 实验揭示, 要保证半定量RT …

Glutamate activates three distinct classes of ionotropic receptors: AMPA, kainate and NMDA.
AMPA receptors (AMPARs) are of particular importance as they mediate the majority of fast …

The insulin-like growth factor binding proteins and pregnancy-associated plasma protein-A
(PAPP-A) within the future dominant follicle is a critical part of selection of one follicle. This …