In humans, nearly two meters of genomic material must be folded to fit inside each
micrometer-scale cell nucleus while remaining accessible for gene transcription, DNA …

Transient complexes are crucial for diverse biological processes such as biochemical
pathways and signaling cascades in the cell. Here, we give an overview of the transient …

Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic
memory in higher eukaryotes. Here, we systematically mapped the human PcG complexome …

Posttranslational modification of histones has emerged as a key regulatory signal in
eukaryotic gene expression. Recent genetic and biochemical studies link H3-lysine 9 (H3 …

We have examined posttranslational regulation of clock proteins in mouse liver in vivo. The
mouse PERIOD proteins (mPER1 and mPER2), CLOCK, and BMAL1 undergo robust …

DNA methylation plays an important role in mammalian development and correlates with
chromatin-associated gene silencing. The recruitment of MeCP2 to methylated CpG …

The activities of several mRNA processing factors are coupled to transcription through
binding to RNA polymerase II (Pol II). The largest subunit of Pol II contains a repetitive …

The role of Notch signaling in growth/differentiation control of mammalian epithelial cells is
still poorly defined. We show that keratinocyte‐specific deletion of the Notch1 gene results in …

Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein
localization and dynamics. We engineered a specific binder for fluorescent proteins based …

Chromatin and transcriptional processes are among the most intensively studied fields of
biology today. The introduction of chromatin immunoprecipitations (ChIP) represents a major …