In 1996, when Dolly the sheep was born, a new, utopian era was expected to begin. Science
fiction and popular culture instantly threatened the public with shortly upcoming human …

During the past 6 years, considerable advancement has been achieved in experimental
embryology of pigs. This process was mainly generated by the rapidly increasing need for …

This study was undertaken to evaluate the development of equine oocytes in vitro and in
vivo after intracytoplasmic sperm injection (ICSI) with either fresh or frozen-thawed …

Isoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle
caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed …

Porcine handmade cloning (HMC), a simplified alternative of micromanipulation based
traditional cloning (TC) has been developed in multiple phases during the past years, but …

Nuclear transfer (NT) is a complex procedure that requires considerable technical skills.
Over the years attempts have been made to simplify the micromanipulations involved and to …

Correct reprogramming of epigenetic marks in the donor nuclei is crucial for successful
cloning by nuclear transfer. Specific epigenetic modifications, such as repressive histone …

The controversy surrounding the alleged Lamarckian fraud of Paul Kammerer's midwife toad
experiments has intrigued generations of biologists. A re‐examination of his descriptions of …

Contents Somatic cell nuclear transfer (SCNT) was first developed in livestock for the
purpose of accelerating the widespread use of superior genotypes. Although many …

Contents The aim of this study was to evaluate three different cloning strategies in the
domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by …