Background Early application of second-generation sequencing technologies to transcript
quantitation (RNA-seq) has hinted at a vast mammalian transcriptome, including transcripts …

Genome-wide expression studies transformed the field of transcriptomics and made it
feasible to study global gene expression in extraordinary detail. These new methods have …

Endoparasitoid wasps develop at the expense of other insects, leading to their death. Eggs
deposited inside the host body induce an immune response, which results in the formation …

RNA sequencing approaches to transcriptome analysis require a large amount of input total
RNA to yield sufficient mRNA using either poly-A selection or depletion of rRNA. This feature …

Background Assessing the reliability of experimental replicates (or global alterations
corresponding to different experimental conditions) is a critical step in analyzing RNA-Seq …

RNA sequencing has increasingly become an indispensable tool for biological research.
While sequencing costs have fallen dramatically in recent years, the current cost of RNA …

Gene-expression heterogeneity among individual cells determines the fate of a bacterial
population. Here we report the first bacterial single-cell RNA sequencing (RNA-seq), BaSiC …

The subcellular localization and translation of messenger RNA (mRNA) supports functional
differentiation between cellular compartments. In neuronal dendrites, local translation of …

Memory T cells are primed for rapid responses to Ag; however, the molecular mechanisms
responsible for priming remain incompletely defined. CpG methylation in promoters is an …

The changes to the epigenetic landscape in response to Ag during CD4 T cell activation
have not been well characterized. Although CD4 T cell subsets have been mapped globally …