The task of transcribing nuclear genes is shared between three RNA polymerases in
eukaryotes: RNA polymerase (pol) I synthesises the large rRNA, pol II synthesises mRNA …

The assembly of a eucaryotic ribosome requires the synthesis of four ribosomal ribonucleic
acid (RNA) molecules and more than 75 ribosomal proteins. It utilizes all three RNA …

We identified a deletion of a gene encoding a subunit of RNA polymerases I and III,
POLR1D, in an individual with Treacher Collins syndrome (TCS). Subsequently, we …

Here, we report the design, construction, and characterization of a tRNA neochromosome, a
designer chromosome that functions as an additional, de novo counterpart to the native …

Protein complexes are assemblies of subunits that have co-evolved to execute one or many
coordinated functions in the cellular environment. Functional annotation of mammalian …

Publisher Summary This chapter describes the In Vitro mutagenesis and plasmid shuffling in
yeast genes. method for generating mutant alleles uses replicating yeast episomes as a …

We describe a set of replicative, integrative and single‐stranded shuttle vectors constructed
from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast …

We analyzed the effects of suppressor mutations in the U1 snRNA (SNR19) gene from
Saccharomyces cerevisiae on the splicing of mutant pre‐mRNA substrates. The results …

Changes in gene dosage of the YAP1 gene, encoding the yAP-1 transcriptional regulatory
protein, cause profound alterations in cellular drug and metal resistance. Previous studies …

We present homologies between archaeal and eucaryal DNA-dependent RNA polymerase
(RNAP) subunits and transcription factors. The sequences of the Sulfolobus acidocaldarius …