In vitro selection of aptamers and their applications

MC DeRosa, A Lin, P Mallikaratchy… - Nature Reviews …, 2023 - nature.com
The introduction of the in vitro evolution method known as SELEX (systematic evolution of
ligands by exponential enrichment) more than 30 years ago led to the conception of …

Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment

S Qi, N Duan, IM Khan, X Dong, Y Zhang, S Wu… - Biotechnology …, 2022 - Elsevier
Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized
libraries, presenting high affinity and specificity toward targets with similar to antibodies …

Recent trends in biosensors for environmental quality monitoring

S Gavrilaș, CȘ Ursachi, S Perța-Crișan… - Sensors, 2022 - mdpi.com
The monitoring of environmental pollution requires fast, reliable, cost-effective and small
devices. This need explains the recent trends in the development of biosensing devices for …

Engineering synthetic RNA devices for cell control

PB Dykstra, M Kaplan, CD Smolke - Nature Reviews Genetics, 2022 - nature.com
The versatility of RNA in sensing and interacting with small molecules, proteins and other
nucleic acids while encoding genetic instructions for protein translation makes it a powerful …

SELEX: Critical factors and optimization strategies for successful aptamer selection

M Kohlberger, G Gadermaier - Biotechnology and applied …, 2022 - Wiley Online Library
Within the last decade, the application range of aptamers in biochemistry and medicine has
expanded rapidly. More than just a replacement for antibodies, these intrinsically structured …

Methods and applications of in silico aptamer design and modeling

AA Buglak, AV Samokhvalov, AV Zherdev… - International journal of …, 2020 - mdpi.com
Aptamers are nucleic acid analogues of antibodies with high affinity to different targets, such
as cells, viruses, proteins, inorganic materials, and coenzymes. Empirical approaches allow …

Aptasensors versus immunosensors—Which will prevail?

S Arshavsky‐Graham, C Heuer… - Engineering in Life …, 2022 - Wiley Online Library
Since the invention of the first biosensors 70 years ago, they have turned into valuable and
versatile tools for various applications, ranging from disease diagnosis to environmental …

Systematic Study of in Vitro Selection Stringency Reveals How To Enrich High-Affinity Aptamers

O Alkhamis, Y Xiao - Journal of the American Chemical Society, 2022 - ACS Publications
Aptamers are oligonucleotide receptors with great potential for sensing and therapeutic
applications. They are isolated from random libraries through an in vitro method termed …

[HTML][HTML] Development of nucleic acid aptamer-based lateral flow assays: A robust platform for cost-effective point-of-care diagnosis

T Wang, L Chen, A Chikkanna, S Chen, I Brusius… - Theranostics, 2021 - ncbi.nlm.nih.gov
Lateral flow assay (LFA) has made a paradigm shift in the in vitro diagnosis field due to its
rapid turnaround time, ease of operation and exceptional affordability. Currently used LFAs …

Quantitative comparison of capture-SELEX, GO-SELEX, and Gold-SELEX for enrichment of aptamers

Y Ding, J Liu - Analytical Chemistry, 2023 - ACS Publications
Since 1990, numerous methods for aptamer selection have been developed, although a
quantitative comparison of their sequence enrichment is lacking. In this study, we compared …