Unraveling the thousand word picture: an introduction to super-resolution data analysis

A Lee, K Tsekouras, C Calderon, C Bustamante… - Chemical …, 2017 - ACS Publications
Super-resolution microscopy provides direct insight into fundamental biological processes
occurring at length scales smaller than light's diffraction limit. The analysis of data at such …

ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging

M Ovesný, P Křížek, J Borkovec, Z Švindrych… - …, 2014 - academic.oup.com
ThunderSTORM is an open-source, interactive and modular plug-in for ImageJ designed for
automated processing, analysis and visualization of data acquired by single-molecule …

Measuring image resolution in optical nanoscopy

RPJ Nieuwenhuizen, KA Lidke, M Bates, DL Puig… - Nature …, 2013 - nature.com
Resolution in optical nanoscopy (or super-resolution microscopy) depends on the
localization uncertainty and density of single fluorescent labels and on the sample's spatial …

MemBright: a family of fluorescent membrane probes for advanced cellular imaging and neuroscience

M Collot, P Ashokkumar, H Anton, E Boutant… - Cell chemical …, 2019 - cell.com
The proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the
cell. Herein, we developed MemBright, a family of six cyanine-based fluorescent turn-on PM …

Optical nanoscopy: from acquisition to analysis

TJ Gould, ST Hess, J Bewersdorf - Annual review of biomedical …, 2012 - annualreviews.org
Recent advances in far-field microscopy have demonstrated that fluorescence imaging is
possible at resolutions well below the long-standing diffraction limit. By exploiting …

Wavelet analysis for single molecule localization microscopy

I Izeddin, J Boulanger, V Racine, CG Specht… - Optics express, 2012 - opg.optica.org
Localization of single molecules in microscopy images is a key step in quantitative single
particle data analysis. Among them, single molecule based super-resolution optical …

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

L Lin, Y Liu, F Xu, J Huang, TF Daugaard… - …, 2018 - academic.oup.com
Background Fusion of DNA methyltransferase domains to the nuclease-deficient clustered
regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has …

Super-resolution surface-enhanced Raman scattering imaging of single particles in cells

CDL De Albuquerque, ZD Schultz - Analytical chemistry, 2020 - ACS Publications
The ability to locate and identify molecular interactions in cells has significant importance for
understanding protein function and molecular biology. Functionalized metallic nanoparticles …

easySTORM: a robust, lower‐cost approach to localisation and TIRF microscopy

K Kwakwa, A Savell, T Davies, I Munro… - Journal of …, 2016 - Wiley Online Library
TIRF and STORM microscopy are super‐resolving fluorescence imaging modalities for
which current implementations on standard microscopes can present significant complexity …

Development of a new approach for low-laser-power super-resolution fluorescence imaging

J Chung, U Jeong, D Jeong, S Go, D Kim - Analytical Chemistry, 2021 - ACS Publications
The development of super-resolution fluorescence microscopy over the past decade has
drastically improved the resolution of light microscopy to∼ 10 nm. Stochastic optical …