High-throughput sequencing-based methods and their applications in the study of
transcriptomes have revolutionized our understanding of alternative splicing. Networks of …

The discovery of introns over four decades ago revealed a new vision of genes and their
interrupted arrangement. Throughout the years, it has appeared that introns play essential …

Pre-mRNA processing steps are tightly coordinated with transcription in many organisms. To
determine how co-transcriptional splicing is integrated with transcription elongation and 3 …

Chimonanthus salicifolius, a member of the Calycanthaceae of magnoliids, is one of the
most famous medicinal plants in Eastern China. Here, we report a chromosome‐level …

In single-cell and single-nucleus RNA sequencing, the coexistence of nascent
(unprocessed) and mature (processed) mRNA poses challenges in accurate read mapping …

Removal of introns by pre-mRNA splicing is fundamental to gene function in eukaryotes.
However, understanding the mechanism by which exon-intron boundaries are defined …

Splicing is a highly regulated process critical for proper pre-mRNA maturation and the
maintenance of a healthy cellular environment. Splicing events are impacted by ongoing …

Recursive splicing is a non-canonical splicing mechanism in which an intron is removed in
segments via multiple splicing reactions. Relatively few recursive splice sites have been …

In single-cell and single-nucleus RNA sequencing (RNA-seq), the coexistence of nascent
(unprocessed) and mature (processed) messenger RNA (mRNA) poses challenges in …

Background The removal of introns occurs through the splicing of a 5′ splice site (5′ ss)
with a 3′ splice site (3′ ss). These two elements are recognized by distinct components of …