Recent advances in cellular profiling have demonstrated substantial heterogeneity in the
behaviour of cells once deemed'identical', challenging fundamental notions of …

Background In recent years, there has been a surge in the development and application of
single-cell genomics, transcriptomics, proteomics, and metabolomics. The metabolome is …

To enable the detection of expression signatures specific to individual cells, we developed
PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript …

In prokaryotes and eukaryotes, most genes appear to be transcribed during short periods
called transcriptional bursts, interspersed by silent intervals. We describe how such bursts …

The genetic circuits that regulate cellular functions are subject to stochastic fluctuations, or
'noise', in the levels of their components. Noise, far from just a nuisance, has begun to be …

Gene expression is a fundamentally stochastic process, with randomness in transcription
and translation leading to cell-to-cell variations in mRNA and protein levels. This variation …

Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …

To measure cell-to-cell variation in protein-mediated functions, we developed an approach
to conduct∼ 103 concurrent single-cell western blots (scWesterns) in∼ 4 h. A microscope …

Gene expression occurs either as an episodic process, characterized by pulsatile bursts, or
as a constitutive process, characterized by a Poisson-like accumulation of gene products. It …

One of the major challenges in modern biology is to understand how the molecular
components of a living cell operate in a highly noisy environment. What are the specific …