Alterations in the regulation of gene expression are frequently associated with
developmental diseases or cancer. Transcription activation is a key phenomenon in the …

Efficient sorting of the material internalized by endocytosis is essential for key cellular
functions and represents a, if not the, major trafficking pathway in mammalian cells …

A critical component in the interpretation of systems-level studies is the inference of enriched
biological pathways and protein complexes contained within OMICs datasets. Successful …

Chinese hamster ovary (CHO)–derived cell lines are the preferred host cells for the
production of therapeutic proteins. Here we present a draft genomic sequence of the CHO …

Retroviruses have long been a fertile model for discovering host–pathogen interactions and
their associated biological principles and processes. These advances have not only …

Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled
genome-wide functional screens in cultured cells and in vivo in model organisms. These …

High-content screening (HCS), which combines automated fluorescence microscopy with
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …

The Mediator complex is a multi-subunit assembly that appears to be required for regulating
expression of most RNA polymerase II (pol II) transcripts, which include protein-coding and …

The HIV-1 capsid is involved in all infectious steps from reverse transcription to integration
site selection, and is the target of multiple host cell and pharmacologic ligands. However …

Loss-of-function genetic screens in model organisms have elucidated numerous biological
processes, but the diploid genome of mammalian cells has precluded large-scale gene …