▪ Abstract Two-photon fluorescence microscopy is one of the most important recent
inventions in biological imaging. This technology enables noninvasive study of biological …

The brain is complex and dynamic. The spatial scales of interest to the neurobiologist range
from individual synapses (∼ 1 μm) to neural circuits (centimeters); the timescales range from …

Microcirculation (2010) 17, 206–225. doi: 10.1111/j. 1549‐8719.2010. 00029. x Abstract
Intravital imaging techniques have provided unprecedented insight into tumor …

Simultaneously measuring the activities of all neurons in a mammalian brain at millisecond
resolution is a challenge beyond the limits of existing techniques in neuroscience. Entirely …

Intravital microscopy coupled with chronic animal window models has provided stunning
insight into tumor pathophysiology, including gene expression, angiogenesis, cell adhesion …

Laser microscopy has generally poor temporal resolution, because of the serial scanning of
each pixel. This is a significant problem for imaging or optically manipulating neural circuits …

Two-photon microscopy is a mainstay technique for imaging in scattering media and
normally provides frame-acquisition rates of~ 10–30 Hz. To track high-speed phenomena …

Despite decades of evidence for functional plasticity in the adult brain, the role of structural
plasticity in its manifestation remains unclear. To examine the extent of neuronal remodeling …

We review the current state of multiphoton microscopy. In particular, the requirements and
limitations associated with high-speed multiphoton imaging are considered. A description of …

Intravital microscopy has provided unprecedented insights into tumor pathophysiology,
including angiogenesis and the microenvironment. Tumor vasculature shows an abnormal …