Gases like H2, N2, CO2, and CO are increasingly recognized as critical feedstock in “green”
energy conversion and as sources of nitrogen and carbon for the agricultural and chemical …

The reaction takes place at a specialized metal center that dramatically increases the acidity
of H2 and leads to a heterolytic splitting of the molecule which is strongly accelerated by the …

Abstract The [FeFe]-and [NiFe]-hydrogenases catalyze the formal interconversion between
hydrogen and protons and electrons, possess characteristic non-protein ligands at their …

[FeFe]-hydrogenases catalyze the reversible reduction of protons to molecular hydrogen
with extremely high efficiency. The active site (“H-cluster”) consists of a [4Fe–4S] H cluster …

S-Adenosylmethionine (SAM, also known as AdoMet) radical enzymes use SAM and a [4Fe-
4S] cluster to catalyze a diverse array of reactions. They adopt a partial triose-phosphate …

[FeFe]-hydrogenases are to date the only enzymes for which it has been demonstrated that
the native inorganic binuclear cofactor of the active site Fe2 (adt)(CO) 3 (CN) 2 (adt …

Conspectus The seeds for recognition of the vast superfamily of radical S-adenosyl-l-
methionine (SAM) enzymes were sown in the 1960s, when Joachim Knappe found that the …

Current textbook knowledge holds that the structural scope of ribosomal biosynthesis is
based exclusively on α-amino acid backbone topology. Here we report the genome-guided …

In Escherichia coli, hydrogen metabolism plays a prominent role in anaerobic physiology.
The genome contains the capability to produce and assemble up to four [NiFe] …

A vast number of enzymes are now known to belong to a superfamily known as radical SAM,
which all contain a [4Fe–4S] cluster ligated by three cysteine residues. The remaining …