Therapeutic enzymes are valuable biopharmaceuticals in various biomedical applications.
They have been successfully applied for fibrinolysis, cancer treatment, enzyme replacement …

Highlights•Enzymes are increasingly used in technological applications but often require
prior optimization.•Computational tools save time and effort in protein engineering …

Abstract Machine learning could enable an unprecedented level of control in protein
engineering for therapeutic and industrial applications. Critical to its use in designing …

The majority of naturally occurring proteins have evolved to function under mild conditions
inside the living organisms. One of the critical obstacles for the use of proteins in …

Abstract Enzymes offering chemo-, regio-, and stereoselectivity enable the asymmetric
synthesis of high-value chiral molecules. Unfortunately, the drawback that naturally …

The drug discovery and development (DDD) process in pursuit of novel drug candidates is a
challenging procedure requiring lots of time and resources. Therefore, computer-aided drug …

Low catalytic activity is a key factor limiting the widespread application of type II L-
asparaginase (ASNase) in the food and pharmaceutical industries. In this study, smart …

Trends in the dynamically developing application of biocatalysis for the synthesis and
modification of polymers over the past 5 years are considered, with an emphasis on the …

Cytochrome P450 CYP153A M. aq from Marinobacter aquaeolei serves as a model enzyme
for the terminal (ω-) hydroxylation of medium-to long-chain fatty acids. We have engineered …

Biocatalysis is a thriving field of research because of the vast, environmentally friendly
impact that enzymes have on industry and biotechnology. However, in most production …